Prokopiw I, Dinda P K, Beck I T
Department of Medicine, Queen's University, Hotel Deiu Hospital, Kingston, Ontario, Canada.
Am J Physiol. 1989 Mar;256(3 Pt 1):G451-7. doi: 10.1152/ajpgi.1989.256.3.G451.
To assess the validity of repeated blood flow measurements using the microsphere technique, the apparent blood flows in the anatomic layers of the jejunum were determined from the entrapment of 9-, 11.5-, and 17-micron microspheres at 1.5, 15, 30, and 60 min after their injection. The entrapment of 17-micron spheres in the mucosa plus submucosa and in the muscularis propria remained similar at all times, but these spheres migrated (P less than 0.01) from the submucosa to the mucosa. By 1.5 min, 5 +/- 2% of 11.5-micron spheres had shunted, but no subsequent shunting was observed. No migration of 11.5-micron spheres from the mucosa, submucosa or the muscularis was observed. The shunting of 9-micron spheres from the whole wall increased from 19 +/- 4% at 1.5 min to 40 +/- 4% at 60 min (P less than 0.001). These data suggest that 17-micron spheres can only fractionate the blood flow of the whole wall into that of the mucosa plus submucosa and that of the muscularis propria, while 11.5-micron spheres may measure fractional flow to the submucosa separately. The continued washout of 9-micron microspheres precludes their use for repeated blood flow measurements.
为评估使用微球技术进行重复血流测量的有效性，在注射9微米、11.5微米和17微米微球后1.5分钟、15分钟、30分钟和60分钟，通过微球滞留情况测定空肠各解剖层的表观血流。17微米微球在黏膜加黏膜下层和固有肌层的滞留情况在各个时间点均保持相似，但这些微球从黏膜下层迁移至黏膜层（P<0.01）。到1.5分钟时，11.5微米微球有5±2%发生分流，但随后未观察到进一步分流。未观察到11.5微米微球从黏膜、黏膜下层或肌层发生迁移。9微米微球从整个肠壁的分流率从1.5分钟时的19±4%增加至60分钟时的40±4%（P<0.001）。这些数据表明，17微米微球只能将整个肠壁的血流分为黏膜加黏膜下层的血流和固有肌层的血流，而11.5微米微球可能可单独测量至黏膜下层的分流血流。9微米微球持续被冲走，因此不能用于重复血流测量。
