Breuer R, Christensen T G, Niles R M, Stone P J, Snider G L
Pulmonary Center, Boston University School of Medicine, Massachusetts.
Am Rev Respir Dis. 1989 Mar;139(3):779-82. doi: 10.1164/ajrccm/139.3.779.
It is known that human neutrophil elastase (HNE) treatment of hamster tracheal explants causes the release of glycoconjugates, most of which appear to have the characteristics of mucus glycoproteins. This study was designed to determine the origin of HNE-induced glycoconjugate release from 1-day-old cultures of adult hamster trachea. After confirming that HNE treatment released glycoconjugates from cultures labeled with tritiated glucosamine, light microscopic autoradiograms and electron micrographs were prepared. Untreated cultures and cultures treated with inactivated HNE served as controls. HNE treatment caused a 40 to 50% decrease in the silver grain count on the external apical surfaces of secretory cells (p less than 0.05) and ciliated cells (p less than 0.01). Silver grain counts in secretory and ciliated cell cytoplasm, submucosa, and nontissue background were not significantly different from controls. The percentage of nongranulated secretory cells and the number of secretory granules in granulated secretory cells were similar in the HNE-treated and untreated controls. There was no evidence of constitutive release of radiolabeled glycoproteins, or of discharge of secretory granules from the secretory cells. We conclude that HNE releases mucins and other glycoconjugates from the external surfaces of both secretory and ciliated cells in tracheal organ culture.
已知用人中性粒细胞弹性蛋白酶（HNE）处理仓鼠气管外植体可导致糖缀合物释放，其中大部分似乎具有黏液糖蛋白的特征。本研究旨在确定成年仓鼠气管1日龄培养物中HNE诱导的糖缀合物释放的来源。在用氚标记的葡糖胺标记培养物后，确认HNE处理可释放糖缀合物，然后制备了光学显微镜放射自显影片和电子显微照片。未处理的培养物和用灭活的HNE处理的培养物作为对照。HNE处理导致分泌细胞（p<0.05）和纤毛细胞（p<0.01）外顶端表面的银粒计数减少40%至50%。分泌细胞和纤毛细胞胞质、黏膜下层及非组织背景中的银粒计数与对照无显著差异。在HNE处理组和未处理的对照组中，无颗粒分泌细胞的百分比以及颗粒化分泌细胞中分泌颗粒的数量相似。没有证据表明存在放射性标记糖蛋白的组成性释放，也没有证据表明分泌细胞释放分泌颗粒。我们得出结论，在气管器官培养中，HNE可从分泌细胞和纤毛细胞的外表面释放黏蛋白和其他糖缀合物。
