Furutani Y, Nomura H, Notake M, Oyamada Y, Fukui T, Yamada M, Larsen C G, Oppenheim J J, Matsushima K
Research Laboratories, Dainippon Pharmaceutical Co. Ltd., Suita/Osaka, Japan.
Biochem Biophys Res Commun. 1989 Feb 28;159(1):249-55. doi: 10.1016/0006-291x(89)92430-3.
cDNA clones having a nucleotide sequence encoding a human monocyte chemotactic and activating factor (MCAF) were isolated and sequenced. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. The amino acid sequence of MCAF showed 25-55% homology with other members of an inducible cytokine family, including macrophage inflammatory protein and some putative polypeptide mediators known as JE, LD78, RANTES and TCA-3. This suggests that MCAF is a member of family of factors involved in immune and inflammatory responses.
分离并测序了具有编码人单核细胞趋化激活因子（MCAF）核苷酸序列的cDNA克隆。从核苷酸序列推导的氨基酸序列显示，MCAF前体的一级结构由23个氨基酸残基的假定信号肽序列和76个氨基酸残基的成熟MCAF序列组成。MCAF的氨基酸序列与诱导性细胞因子家族的其他成员具有25%-55%的同源性，这些成员包括巨噬细胞炎性蛋白以及一些被称为JE、LD78、RANTES和TCA-3的假定多肽介质。这表明MCAF是参与免疫和炎症反应的因子家族成员。
