University Health Network, Toronto General Hospital, Ontario M5G2C4, Canada.
Dahl-Chase Diagnostic Services in Bangor, Bangor, Maine.
Cytometry B Clin Cytom. 2018 Jan;94(1):23-48. doi: 10.1002/cyto.b.21610.
Since publication in 2010 of the International Clinical Cytometry Society (ICCS) Consensus Guidelines for detection of Paroxysmal nocturnal hemoglobinuria (PNH) by flow cytometery, a great deal of work has been performed to develop, optimize, and validate a number of high-sensitivity assays to detect PNH phenotypes in both red blood cells (RBCs) and white blood cells (WBCs, neutrophils, and monocytes). This section (Part 2) of the updated ICCS PNH Consensus Guidelines will focus on specific instrument setup for these PNH assays, the identification and proper testing of appropriate antibody conjugates and combinations therof, and basic assay design. © 2017 International Clinical Cytometry Society.
自 2010 年国际临床细胞化学学会（ICCS）发布流式细胞术检测阵发性夜间血红蛋白尿症（PNH）的共识指南以来，已经开展了大量工作来开发、优化和验证许多高灵敏度检测方法，以检测红细胞（RBC）和白细胞（WBC、中性粒细胞和单核细胞）中的 PNH 表型。本更新的 ICCS PNH 共识指南的这一部分（第 2 部分）将重点介绍这些 PNH 检测的特定仪器设置、合适的抗体缀合物的识别和正确测试及其组合，以及基本的检测设计。© 2017 年国际临床细胞化学学会。
