Galey D, Toumane A, Durkin T, Jaffard R
Laboratoire de Psychophysiologie, C.N.R.S., Talence, France.
Behav Brain Res. 1989 Mar 1;32(2):163-72. doi: 10.1016/s0166-4328(89)80082-8.
Dopaminergic afferents to the septum mediate a tonic and trans-synaptic inhibitory control on the cholinergic neurones of the septo-hippocampal pathway. Lesion of these afferents using 6-hydroxydopamine (6-OHDA) results in a chronic and specific increase of hippocampal cholinergic activity in mice. The consequence of this in vivo modulation of hippocampal cholinergic activity on the acquisition of both a spatial discrimination and a working memory (delayed non-matching to place) task in an 8-arm radial maze by C57BL/6 mice were investigated. Combined neurochemical and behavioural analyses revealed significant correlations between hippocampal sodium-dependent high-affinity choline uptake activation induced by testing and performance measures. In the first experiment 6-OHDA-treated mice compared to control and vehicle-injected mice showed a transient (day 2) but significant facilitation of their spatial discrimination performance which appears to be better related to the working but not to the reference memory component of the task. This hypothesis is strengthened by the results of the second experiment which shows an amelioration of working memory performance when the septo-hippocampal cholinergic pathway is specifically activated in vivo.
多巴胺能传入纤维至隔区介导对隔-海马通路胆碱能神经元的紧张性和跨突触抑制性控制。用6-羟基多巴胺（6-OHDA）损伤这些传入纤维会导致小鼠海马胆碱能活性慢性且特异性增加。研究了这种体内海马胆碱能活性调节对C57BL/6小鼠在八臂放射状迷宫中获取空间辨别和工作记忆（延迟位置不匹配）任务的影响。联合神经化学和行为分析揭示了测试诱导的海马钠依赖性高亲和力胆碱摄取激活与行为表现指标之间的显著相关性。在第一个实验中，与对照和注射赋形剂的小鼠相比，6-OHDA处理的小鼠表现出短暂（第2天）但显著的空间辨别能力促进，这似乎与任务的工作记忆成分而非参考记忆成分更好相关。第二个实验的结果强化了这一假设，该实验表明当隔-海马胆碱能通路在体内被特异性激活时，工作记忆表现得到改善。
