他达拉非对大鼠缺血再灌注的保护作用。

Tadalafil protector effect during ischemia-reperfusion in rats.

作者信息

Wietzikoski Eduardo Gabriel Gerber, Foiatto Julio Cesar, Czeczko Nicolau Gregori, Malafaia Osvaldo, Koleski Fernando Cesar, Mierzwa Tiago Cesar, Gomes Regina de Paula Xavier

机构信息

Fellow Master degree, Postgraduate Program in Surgery Principles, Medical Research Institute, Faculdade Evangélica do Paraná (FEPAR), Curitiba-PR, Brazil. Conception, design, intellectual and scientific content of the study; manuscript preparation.

Associate Professor, Postgraduate Program in Principles of Surgery, Medical Research Institute, FEPAR, Curitiba-PR, Brazil. Intellectual and scientific content of the study, interpretation of data, critical revision.

出版信息

Acta Cir Bras. 2017 Nov;32(11):973-983. doi: 10.1590/s0102-865020170110000009.

DOI:10.1590/s0102-865020170110000009

PMID:29236802

Abstract

PURPOSE

To evaluate histological parameters in rat renal tissue after tadalafil use during hot ischemia for 45 minutes and reperfusion for 24 hours.

METHODS

Twenty rats were divided into 2 groups. In the experimental group 10 mg/kg of tadalafil was used per gavage before the procedure. All cases underwent left partial nephrectomy, followed by 45 minutes of warm ischemia. Left nephrectomy of the remaining kidney was performed after 24 hours from the initial procedure. The histological parameters analyzed were: detachment of tubular cells, accumulation of desquamated cells in the proximal tubule, loss of brush border, tubular cylinders, interstitial edema, leukocyte infiltration, capillary congestion, vacuolization, tubular dilatation, necrosis and collapse of the capillary tuft.

RESULTS

Two rats from each group died and were excluded from the study. Tadalafil significantly reduced leukocyte infiltration (p = 0.036). The remaining histological parameters did not show statistical difference between the groups.

CONCLUSION

The use of tadalafil during warm ischemia and reperfusion demonstrates statistically significant reduction of leukocyte infiltration in the renal interstitium.

摘要

目的

评估他达拉非在大鼠热缺血45分钟及再灌注24小时期间使用后肾组织的组织学参数。

方法

将20只大鼠分为2组。实验组在手术前经口灌胃给予10mg/kg他达拉非。所有病例均行左半肾切除术，随后进行45分钟的热缺血。初始手术后24小时对剩余肾脏进行左肾切除术。分析的组织学参数包括：肾小管细胞脱落、近端小管中脱落细胞的积聚、刷状缘丧失、肾小管管型、间质水肿、白细胞浸润、毛细血管充血、空泡化、肾小管扩张、毛细血管袢坏死和塌陷。