Chosun University, School of Dentistry, Department of Oral and Maxillofacial Surgery, 375, SeoSukDong, DongGu, Gwangju 501-759, Republic of Korea.
Braz Oral Res. 2017 Dec 7;31:e99. doi: 10.1590/1807-3107bor-2017.vol31.0099.
The aim of the present study was to evaluate the possible use of a commercial absorbed collagen sponge and bone morphogenetic protein (BMP) for the prevention of bisphosphonate-related osteonecrosis of the jaw (BRONJ) in rats. Twenty rats received intraperitoneal injections of 0.1-mg/kg of zoledronic acid three times a week for eight weeks before the extraction of both maxillary first molars after eight weeks. A collagen sponge (experimental group 1) and a collagen sponge with recombinant human BMP-2 (experimental group 2) were applied to the right extraction sockets of ten rats each. The 20 left extraction sockets (control groups 1 and 2) were left unprotected. After eight weeks, all rats were euthanized. Macroscopic analysis, micro-computed tomography (CT) analysis, and histological analysis were performed. There was a significant difference in the bone density between the control and experimental groups on micro-CT analysis. Impaired healing of the extraction sockets, indicating BRONJ, was observed in 80% of control group 1, 90% of control group 2, 30% of experimental group 1, and 20% of experimental group 2. The collagen sponge with/without BMP used for protecting the extraction socket had the potential for a positive effect in reducing the incidence of bisphosphonate-related osteonecrosis of the jaw in rats.
本研究旨在评估商业吸收性胶原海绵和骨形态发生蛋白（BMP）在预防大鼠抗二膦酸盐相关性颌骨坏死（BRONJ）中的可能用途。20 只大鼠在第 8 周前每周接受 3 次腹腔注射 0.1mg/kg唑来膦酸 8 周，然后拔除上颌第一磨牙。将胶原海绵（实验组 1）和含有重组人 BMP-2 的胶原海绵（实验组 2）分别应用于 10 只大鼠的右侧拔牙窝。20 个左侧拔牙窝（对照组 1 和 2）未作任何保护。8 周后，所有大鼠被安乐死。进行了大体分析、微计算机断层扫描（CT）分析和组织学分析。在微 CT 分析中，对照组和实验组之间的骨密度有显著差异。在对照组 1 中，80%、对照组 2 中 90%、实验组 1 中 30%和实验组 2 中 20%的大鼠拔牙窝愈合受损，表明出现 BRONJ。使用含/不含 BMP 的胶原海绵保护拔牙窝可能具有降低大鼠抗二膦酸盐相关性颌骨坏死发生率的积极作用。
