在大鼠胃酸分泌刺激实验模型中，氢硫化钠上调胃黏膜中转化生长因子-α（TGF-α）的mRNA和蛋白表达。
Sodium hydrosulfide upregulates mRNA and protein expression of TGF-α in gastric mucosa in experimental model of stimulated gastric acid secretion in rats.
作者信息
Akbari Ghaidafeh, Mard Seyed Ali, Khoshnam Seyed Esmaeil, Ahmadi Iraj
机构信息
Physiology Research Center (PRC), Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Research Center for Infectious Diseases of Digestive System [Alimentary Tract Research Center], Physiology Research Center (PRC), Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
出版信息
Iran J Basic Med Sci. 2017 Dec;20(12):1385-1389. doi: 10.22038/IJBMS.2017.9597.
OBJECTIVES
Transforming growth factor alpha (TGF-α) has been shown to modulate the gastric acid secretion. Therefore, the aim of the present study was to investigate the effect of sodium hydrosulfide (NaHS) on TGF-α expression in gastric mucosa in rats.
MATERIALS AND METHODS
Eighteen rats were randomly divided into 3 groups (6 per group). To determine the effect of NaHS on gene and protein expression of TGF-α in gastric mucosa in response to gastric acid, the acid output induced by gastric distension. At the end of experiment, rats were euthanized by anesthetics, and gastric effluents, in addition to mucosa were collected to measure the pH of gastric effluents and to quantify protein and gene expression of TGF-α.
RESULTS
The stimulated gastric acid upregulated expression levels of TGF-α in gastric mucosa. These levels were higher in animals pretreated with NaHS.
CONCLUSION
TGF-α upregulatory effect of sodium hydrosulfate implied that TGF-α is involved in the acid inhibitory effect of NaHS.
目的
转化生长因子α（TGF-α）已被证明可调节胃酸分泌。因此，本研究的目的是探讨氢硫化钠（NaHS）对大鼠胃黏膜中TGF-α表达的影响。
材料与方法
18只大鼠随机分为3组（每组6只）。为了确定NaHS对胃扩张诱导的胃酸分泌时胃黏膜中TGF-α基因和蛋白表达的影响。实验结束时，用麻醉剂使大鼠安乐死，收集胃流出物及胃黏膜，以测量胃流出物的pH值，并定量TGF-α的蛋白和基因表达。
结果
刺激胃酸上调了胃黏膜中TGF-α的表达水平。在用NaHS预处理的动物中，这些水平更高。
结论
氢硫酸钠对TGF-α的上调作用表明TGF-α参与了NaHS的抑酸作用。
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