Li Y, Xu D B, Wang H J
Department of Burns and Plastic Surgery, Qinghai University Affiliated Hospital, Xining 810001, China.
Zhonghua Shao Shang Za Zhi. 2016 Jul 20;32(7):408-12. doi: 10.3760/cma.j.issn.1009-2587.2016.07.005.
To analyze the effects of exogenous hydrogen sulfide on the secretion of growth factors basic fibroblast growth factor (bFGF) and transforming growth factor β1 (TGF-β1), as well as inflammatory mediators tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β) in macrophages of deep partial-thickness burn wound in rats.
Seventy-eight SD rats were divided into normal control group (n=6), pure burn group, sodium hydrosulfide group, propargylglycine (PPG) group, and sodium hydrosulfide+ PPG group according to the random number table, with 18 rats in each of the latter four groups. Rats in normal control group did not receive any treatment, while rats in the other four groups were inflicted with 5% total burn surface area deep partial-thickness scald (hereinafter referred to as burn) on the back. Immediately after burn, rats in pure burn group, sodium hydrosulfide group, and group PPG were intraperitoneally injected with saline 2 mL/kg, sodium hydrosulfide 56 μmol/kg, and PPG 45 mg/kg respectively, while those in sodium hydrosulfide+ PPG group were intraperitoneally injected with sodium hydrosulfide 56 μmol/kg and PPG 45 mg/kg, once a day till the day before harvesting specimen. Six rats of normal control group fed for one week, and 6 rats from each of the rest four groups on post injury day (PID) 3, 7, 14 were collected respectively. Normal skin on the back of rats in normal control group and tissue in the base of wound of rats in the other four groups were harvested to isolate macrophages, and then the content of bFGF, TGF-β1, TNF-α, and IL-1β in culture supernatant of macrophages was detected with enzyme-linked immunosorbent assay. Data were processed with one-way analysis of variance, analysis of variance of factorial design, and LSD test.
Compared with that of normal control group [(42.6±2.5) and (18±4) pg/mL], the content of bFGF and TGF-β1 in culture supernatant of macrophages of rats in pure burn group was obviously increased at each time point (with P values below 0.01), peaking on PID 14 at (141.6±7.7) and (580±16) pg/mL respectively. Compared with that of pure burn group, the content of bFGF and TGF-β1 in culture supernatant of macrophages of rats in sodium hydrosulfide group was obviously increased at each time point (with P values below 0.01), peaking on PID 14 at (193.7±10.9) and (793±12) pg/mL respectively, while the content of bFGF and TGF-β1 in culture supernatant of macrophages of rats in group PPG was obviously decreased at each time point (with P values below 0.01), reaching the nadir on PID 3 at (62.0±7.1) and (170±10) pg/mL respectively. The content of bFGF and TGF-β1 in culture supernatant of macrophages of rats in sodium hydrosulfide+ PPG group was obviously lower than that of sodium hydrosulfide group but obviously higher than that of group PPG at each time point (with P values below 0.01), peaking on PID 14 at (151.3±9.0) and (579±9) pg/mL respectively. Compared with that of normal control group [(97±6) and (31±6) pg/mL], the content of TNF-α and IL-1β in culture supernatant of macrophages of rats in pure burn group was obviously increased at each time point (with P values below 0.01), peaking on PID 3 at (924±8) and (290±10) pg/mL respectively. Compared with that of pure burn group, the content of TNF-α and IL-1β in culture supernatant of macrophages of rats in sodium hydrosulfide group was obviously decreased at each time point (with P values below 0.01), reaching the nadir on PID 14 at (346±10) and (120±5) pg/mL respectively, while the content of TNF-α and IL-1β in culture supernatant of macrophages of rats in group PPG was obviously increased at each time point (with P values below 0.01), peaking on PID 3 at (1 232±13) and (410±10) pg/mL respectively. The content of TNF-α and IL-1β in culture supernatant of macrophages of rats in sodium hydrosulfide+ PPG group was obviously higher than that of sodium hydrosulfide group but obviously lower than that of group PPG at each time point (with P values below 0.01), reaching the nadir on PID 14 at (488±16) and (144±6) pg/mL respectively.
Supplementation of exogenous hydrogen sulfide in small dosage can increase the secretion of growth factors bFGF and TGF-β1 in macrophages of wound in rats with deep partial-thickness burn in the early stage and reduce the release of inflammatory mediators TNF-α and IL-1β in the meantime, thus affecting the healing of wound.
分析外源性硫化氢对大鼠深Ⅱ度烧伤创面巨噬细胞中生长因子碱性成纤维细胞生长因子（bFGF）和转化生长因子β1（TGF-β1）以及炎性介质肿瘤坏死因子α（TNF-α）和白细胞介素1β（IL-1β）分泌的影响。
将78只SD大鼠按随机数字表法分为正常对照组（n = 6）、单纯烧伤组、氢硫化钠组、炔丙基甘氨酸（PPG）组和氢硫化钠 + PPG组，后四组每组18只。正常对照组大鼠不做任何处理，其余四组大鼠均于背部造成5%体表面积的深Ⅱ度烫伤（以下简称烧伤）。烧伤后即刻，单纯烧伤组、氢硫化钠组和PPG组大鼠分别腹腔注射生理盐水2 mL/kg、氢硫化钠56 μmol/kg、PPG 45 mg/kg，氢硫化钠 + PPG组大鼠腹腔注射氢硫化钠56 μmol/kg和PPG 45 mg/kg，每天1次，直至取材前一天。正常对照组6只大鼠饲养1周，其余四组分别于伤后第3、7、14天各取6只大鼠。取正常对照组大鼠背部正常皮肤及其他四组大鼠创面基底部组织分离巨噬细胞，然后采用酶联免疫吸附测定法检测巨噬细胞培养上清液中bFGF、TGF-β1、TNF-α和IL-1β的含量。数据采用单因素方差分析、析因设计方差分析及LSD检验进行处理。
与正常对照组[（42.6±2.5）和（18±4）pg/mL]比较，单纯烧伤组大鼠巨噬细胞培养上清液中bFGF和TGF-β1含量在各时间点均明显升高（P值均<0.01），于伤后第14天达峰值，分别为（141.6±7.7）和（580±16）pg/mL。与单纯烧伤组比较，氢硫化钠组大鼠巨噬细胞培养上清液中bFGF和TGF-β1含量在各时间点均明显升高（P值均<0.01），于伤后第14天达峰值，分别为（193.7±10.9）和（793±12）pg/mL；而PPG组大鼠巨噬细胞培养上清液中bFGF和TGF-β1含量在各时间点均明显降低（P值均<0.01），于伤后第3天降至最低点，分别为（62.0±7.1）和（170±10）pg/mL。氢硫化钠 + PPG组大鼠巨噬细胞培养上清液中bFGF和TGF-β1含量在各时间点均明显低于氢硫化钠组，但明显高于PPG组（P值均<0.01），于伤后第14天达峰值，分别为（151.3±9.0）和（579±9）pg/mL。与正常对照组[（97±6）和（31±6）pg/mL]比较，单纯烧伤组大鼠巨噬细胞培养上清液中TNF-α和IL-1β含量在各时间点均明显升高（P值均<0.01），于伤后第3天达峰值，分别为（924±8）和（290±10）pg/mL。与单纯烧伤组比较，氢硫化钠组大鼠巨噬细胞培养上清液中TNF-α和IL-1β含量在各时间点均明显降低（P值均<0.01），于伤后第
