Jackson J A, Butterstein G M, Albrecht E D
Department of Obstetrics/Gynecology, University of Maryland School of Medicine, Baltimore 21201.
Biol Reprod. 1989 Jan;40(1):74-80. doi: 10.1095/biolreprod40.1.74.
We have recently provided evidence from studies conducted in vivo that the ovary, particularly by means of estrogen, regulates placental androstenedione (delta 4A) production during the second half of rat pregnancy. In the present study, an incubation system of dispersed rat placental cells was established to determine if estrogen acts directly on the placenta to regulate delta 4A production. Placentas were obtained on Days 14-15 of rat gestation and dispersed in Hanks' Balanced Salt Solution containing 0.1% collagenase, 0.1% hyaluronidase, 0.01% DNase, and 1% fetal calf serum. Placental cells were incubated in Medium 199 for 16 h at 37 degrees C. A time-dependent increase (r = 0.96, p less than 0.05) in the release of delta 4A occurred over the 16-h incubation period. Mean +/- SE formation of the steroid intermediate progesterone (P4) and product delta 4A was 1.17 +/- 0.78 and 1.18 +/- 0.22 ng per 10(7) cells respectively. The addition of 1-10 microM diethylstilbestrol (DES) decreased (p less than 0.05-0.01) delta 4A production, and had no significant effect on P4 or pregnenolone (P5) formation. The percent decrease in delta 4A production was 14.2 +/- 12.9, 30.9 +/- 2.3, and 55.0 +/- 4.4 with 1, 5, and 10 microM DES, respectively. Treatment of placental cells with estradiol (E2) also resulted in a decrease (p less than 0.01) in delta 4A production with no effect on P4 formation. The percent inhibition of delta 4A production was 34.2 +/- 11.1 and 77.3 +/- 5.2 with the addition of 1 microM and 10 microM E2, respectively. E2 (10 microM) produced a concomitant threefold increase (p less than 0.01) in P5 formation.(ABSTRACT TRUNCATED AT 250 WORDS)
我们最近通过体内研究提供了证据，表明卵巢，特别是通过雌激素，在大鼠妊娠后半期调节胎盘雄烯二酮（δ4A）的产生。在本研究中，建立了分散的大鼠胎盘细胞孵育系统，以确定雌激素是否直接作用于胎盘来调节δ4A的产生。在大鼠妊娠第14 - 15天获取胎盘，并将其分散于含有0.1%胶原酶、0.1%透明质酸酶、0.01%脱氧核糖核酸酶和1%胎牛血清的汉克斯平衡盐溶液中。胎盘细胞在199培养基中于37℃孵育16小时。在16小时的孵育期内，δ4A的释放呈时间依赖性增加（r = 0.96，p < 0.05）。每10^7个细胞中类固醇中间体孕酮（P4）和产物δ4A的平均±标准误形成量分别为1.17±0.78和1.18±0.22纳克。添加1 - 10微摩尔己烯雌酚（DES）可降低（p < 0.05 - 0.01）δ4A的产生，对P4或孕烯醇酮（P5）的形成无显著影响。添加1、5和10微摩尔DES时，δ4A产生的降低百分比分别为14.2±12.9、30.9±2.3和55.0±4.4。用雌二醇（E2）处理胎盘细胞也导致δ4A产生降低（p < 0.01），对P4形成无影响。添加1微摩尔和10微摩尔E2时，δ4A产生的抑制百分比分别为34.2±11.1和77.3±5.2。10微摩尔E2使P5的形成伴随增加了三倍（p < 0.01）。（摘要截短于250字）
