Freund T F
MRC Anatomical Neuropharmacology Unit, Department of Pharmacology, Oxford, U.K.
Brain Res. 1989 Jan 30;478(2):375-81. doi: 10.1016/0006-8993(89)91520-5.
Septal axons were visualized in the hippocampus by anterograde transport of Phaseolus vulgaris leucoagglutinin (PHAL) injected into the medial septal region, which contains large numbers of parvalbumin (PV)-immunoreactive somata. A proportion of the PHAL-labelled afferents in the hippocampus were shown to be immunoreactive for PV by immunostaining alternate sections. This population of septohippocampal axons alone was found to be immunoreactive for gamma-aminobutyric acid (GABA) following post-embedding immunogold staining of ultrathin sections cut from the same material.
通过将菜豆白细胞凝集素(PHAL)注入内侧隔区来进行顺行运输,从而在海马体中观察到隔区轴突,内侧隔区包含大量抗小白蛋白(PV)免疫反应性胞体。通过对交替切片进行免疫染色,发现海马体中一部分PHAL标记的传入神经对PV具有免疫反应性。在对同一材料制成的超薄切片进行包埋后免疫金染色后,发现仅这群隔海马轴突对γ-氨基丁酸(GABA)具有免疫反应性。