Department of Otolaryngology, Ji'ning First People's Hospital of Shandong Province, Ji'ning, China.
Eur Rev Med Pharmacol Sci. 2017 Dec;21(23):5378-5385. doi: 10.26355/eurrev_201712_13923.
To detect the expressions of nasopharyngeal carcinoma-associated gene 6 (NGX6) in nasopharyngeal carcinoma cells and tissues, and to investigate the effects of NGX6 on the proliferation and invasion of nasopharyngeal carcinoma cells and the survival of patients.
The human nasopharyngeal carcinoma cells (HONE1) and immortalized human nasopharyngeal epithelial cells (NP69) were selected and cultured. The mRNA and protein expression levels of NGX6 were detected via quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot. The expression of NGX6 in HONE1 was up-regulated using the gene transfection technique. Moreover, the effects of NGX6 on the proliferation and invasion capacities of HONE1 were observed via methyl thiazolyl tetrazolium (MTT) assay and Transwell assay. 50 biopsy tissue specimens of nasopharyngeal carcinoma and 20 non-neoplastic nasopharyngeal biopsy tissue specimens were collected, and the immunohistochemical method was used to detect the protein expression of NGX6 in tumor tissues of patients with esophageal carcinoma. Finally, the follow-up data of patients were recorded, Kaplan-Meier method was used for survival analysis, and the difference in survival rates was detected using the Log-rank test.
The results of qRT-PCR and Western blot showed that the mRNA and protein expressions of NGX6 in HONE1 were significantly lower than those in nasopharyngeal carcinoma cells (NP69). After the overexpression of NGX6, the protein expression of NGX6 in HONE1 was significantly increased, but the proliferation and invasion capacities of HONE1 were significantly decreased. Besides, the immunohistochemical results revealed that the expression of NGX6 in tumor tissues of patients with nasopharyngeal carcinoma was significantly lower than that in normal tissues; the survival analysis showed that the level of NGX6 was positively correlated with the survival and prognosis of patients with nasopharyngeal carcinoma.
NGX6 is lowly expressed in nasopharyngeal carcinoma, and it can inhibit the proliferation and invasion of nasopharyngeal carcinoma cells, whose expression is positively correlated with the survival and prognosis of patients with nasopharyngeal carcinoma.
检测鼻咽癌相关基因 6（NGX6）在鼻咽癌细胞和组织中的表达，探讨 NGX6 对鼻咽癌细胞增殖、侵袭和患者生存的影响。
选择并培养人鼻咽癌细胞（HONE1）和永生化人鼻咽上皮细胞（NP69）。采用实时荧光定量聚合酶链反应（qRT-PCR）和 Western blot 检测 NGX6 的 mRNA 和蛋白表达水平。采用基因转染技术上调 HONE1 中 NGX6 的表达。此外，通过噻唑蓝（MTT）比色法和 Transwell 实验观察 NGX6 对 HONE1 增殖和侵袭能力的影响。收集 50 例鼻咽癌活检组织标本和 20 例非肿瘤性鼻咽活检组织标本，采用免疫组织化学法检测 NGX6 在食管癌患者肿瘤组织中的蛋白表达。最后，记录患者的随访资料，采用 Kaplan-Meier 法进行生存分析，采用 Log-rank 检验检测生存率的差异。
qRT-PCR 和 Western blot 结果显示，HONE1 中 NGX6 的 mRNA 和蛋白表达明显低于鼻咽癌细胞（NP69）。过表达 NGX6 后，HONE1 中 NGX6 的蛋白表达明显增加，但 HONE1 的增殖和侵袭能力明显下降。此外，免疫组织化学结果显示，鼻咽癌患者肿瘤组织中 NGX6 的表达明显低于正常组织；生存分析显示，NGX6 水平与鼻咽癌患者的生存和预后呈正相关。
NGX6 在鼻咽癌中低表达，可抑制鼻咽癌细胞的增殖和侵袭，其表达与鼻咽癌患者的生存和预后呈正相关。
