Department of Gastroenterology, People's Hospital of Weifang, Weifang, Shandong, China.
Eur Rev Med Pharmacol Sci. 2017 Dec;21(23):5506-5514. doi: 10.26355/eurrev_201712_13942.
We aimed at investigating the effects of rapamycin on apoptosis and autophagy of human acute promyelocytic leukemia cell line HL-60, and to preliminarily explore the mechanism of extra medullary infiltration of leukemia cells with human acute promyelocytic leukemia cell line HL-60 as the object of study, providing a theoretical basis for the clinical treatment of leukemia.
After HL-60 cells were cultured in vitro, the effect of rapamycin on proliferation ability of HL-60 cells was determined by methyl thiazolyl tetrazolium (MTT) method, the cell apoptosis ratio was detected by flow cytometer, the change of autophagy after HL-60 cells acted by rapamycin was tested by monodansylcadaverine (MDC) fluorescence staining, the mRNA expression of autophagy-related molecule was detected by polymerase chain reaction (PCR), and the expressions of apoptosis-related protein and autophagy-related protein were determined by Western blotting (WB).
HL-60 cell proliferation could be significantly inhibited by rapamycin (80 μg/mL-640 μg/mL), which was in a dose-dependent manner. HL-60 cell apoptosis ratio and apoptosis-related protein expression were distinctly improved by rapamycin. Cell autophagy level, mRNA expression of autophagy-related molecule and autophagy-related protein expression were remarkably induced by rapamycin.
Rapamycin can induce HL-60 cell apoptosis, which is produced mainly by inducing cell autophagy.
研究雷帕霉素对人急性早幼粒细胞白血病细胞系 HL-60 细胞凋亡和自噬的影响，并初步探讨雷帕霉素对人急性早幼粒细胞白血病细胞系 HL-60 细胞髓外浸润的作用机制，为白血病的临床治疗提供理论依据。
体外培养 HL-60 细胞，采用噻唑蓝（MTT）比色法检测雷帕霉素对 HL-60 细胞增殖能力的影响，流式细胞术检测细胞凋亡率，单丹磺酰尸胺（MDC）荧光染色检测雷帕霉素作用后 HL-60 细胞自噬的变化，聚合酶链反应（PCR）检测自噬相关分子的 mRNA 表达，Western blot（WB）检测凋亡相关蛋白和自噬相关蛋白的表达。
雷帕霉素（80μg/ml-640μg/ml）能显著抑制 HL-60 细胞增殖，呈剂量依赖性。雷帕霉素明显提高 HL-60 细胞凋亡率和凋亡相关蛋白的表达。雷帕霉素显著诱导细胞自噬水平、自噬相关分子的 mRNA 表达和自噬相关蛋白的表达。
雷帕霉素可诱导 HL-60 细胞凋亡，主要通过诱导细胞自噬产生。
