Chouhy Diego, Kocjan Boštjan J, Staheli Jeannette P, Bolatti Elisa M, Hošnjak Lea, Sagadin Martin, Giri Adriana A, Rose Timothy M, Poljak Mario
Grupo Virología Humana, Instituto de Biología Molecular y Celular de Rosario (CONICET), Suipacha 590, 2000 Rosario, Argentina.
Área Virología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, 2000 Rosario, Argentina.
J Gen Virol. 2018 Jan;99(1):109-118. doi: 10.1099/jgv.0.000988. Epub 2017 Dec 15.
A modified pan-PV consensus-degenerate hybrid oligonucleotide primer (CODEHOP) PCR was developed for generic and sensitive detection of a broad-spectrum of human papillomaviruses (HPVs) infecting the cutaneous epithelium. To test the analytical sensitivity of the assay we examined 149 eyebrow hair follicle specimens from immunocompetent male patients. HPV DNA was detected in 60 % (89/149) of analysed eyebrow samples with a total of 48 different HPV sequences, representing 21 previously described HPVs and 27 putative novel HPV types. Evidence for ten novel HPV subtypes and seven viral variants, clustering to three out of five genera containing cutaneous HPVs, was also obtained. Thus, we have shown that the modified pan-PV CODEHOP PCR assay is able to identify multiple HPV types, even from different genera, in the same clinical sample. Overall, these results demonstrate that the pan-PV CODEHOP PCR is an excellent tool for screening and identification of novel cutaneous HPVs, even in samples with low viral loads.
我们开发了一种改良的泛乳头瘤病毒（PV）一致序列简并杂交寡核苷酸引物（CODEHOP）PCR方法，用于广泛且灵敏地检测感染皮肤上皮的广谱人乳头瘤病毒（HPV）。为测试该检测方法的分析灵敏度，我们检测了149例免疫功能正常男性患者的眉部毛囊标本。在60%（89/149）的分析眉部样本中检测到HPV DNA，共有48种不同的HPV序列，代表21种先前描述的HPV和27种推定的新型HPV类型。还获得了10种新型HPV亚型和7种病毒变体的证据，它们聚集在包含皮肤HPV的5个属中的3个属中。因此，我们已经表明，改良的泛PV CODEHOP PCR检测方法能够在同一临床样本中鉴定多种HPV类型，甚至是来自不同属的类型。总体而言，这些结果表明，泛PV CODEHOP PCR是筛查和鉴定新型皮肤HPV的优秀工具，即使在病毒载量较低的样本中也是如此。
