Tao Yanfei, Xie Shuyu, Zhu Yufei, Chen Dongmei, Pan Yuanhu, Wang Xu, Liu Zhenli, Huang Lingli, Peng Dapeng, Yuan Zonghui
National Reference Laboratory of Veterinary Drug Residues (HZAU)/MOA Key Laboratory of Food Safety Evaluation, Huazhong Agricultural University, Hongshan, Shizi shan Street No. 1, Wuhan 430070, China.
J Chromatogr Sci. 2018 Mar 1;56(3):285-291. doi: 10.1093/chromsci/bmx096.
A quantitative LC-MS/MS method has been developed for simultaneous determination of bacitracin A, bacitracin B, colistin A, colistin B and virginiamycin M1 in feed. This rapid simple and effective extraction method was based on matrix solid-phase dispersion. Qualitative and quantitative analyses were performed by LC-ESI-MS/MS. CCβ of polypeptide antibiotics upon the method ranged from 9.6 to 15.8 μg kg-1 and 19.4 to 27.5 μg kg-1, respectively. The limit of quantification of polypeptide antibiotics was 25 μg kg-1 in feed samples. The recoveries of polypeptide antibiotics spiked in feed samples at a concentration range of 25-100 μg kg-1 were found above 75.9-87.9% with relative standard deviations within days less than 15.7% and between days less than 20.6%. This rapid and reliable method can be used to efficiently separate, characterize and quantify the residues of polypeptide antibiotics in feed with advantages of simple pretreatment and environmental friendly.
已开发出一种定量液相色谱-串联质谱法，用于同时测定饲料中的杆菌肽A、杆菌肽B、黏菌素A、黏菌素B和维吉尼亚霉素M1。这种快速、简单且有效的提取方法基于基质固相分散。通过液相色谱-电喷雾电离-串联质谱进行定性和定量分析。该方法对多肽类抗生素的定量限分别为9.6至15.8 μg kg-1和19.4至27.5 μg kg-1。饲料样品中多肽类抗生素的定量限为25 μg kg-1。在浓度范围为25-100 μg kg-1的饲料样品中添加多肽类抗生素的回收率高于75.9-87.9%，日内相对标准偏差小于15.7%，日间相对标准偏差小于20.6%。这种快速可靠的方法可用于高效分离、表征和定量饲料中多肽类抗生素的残留，具有预处理简单和环境友好的优点。
