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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Department of Zoology, Iowa State University, Ames 50011.

Comp Biochem Physiol B. 1989;92(2):251-4. doi: 10.1016/0305-0491(89)90274-5.

Protein kinase C is considered to be ubiquitous in tissues and organs; however, its isolation and characterization have been principally with adult mammalian tissues. 2. There is increasing evidence for the importance of this enzyme during early development. 3. In this study, protein kinase C has been identified and partially characterized in cytosolic fraction from sea urchin eggs. 4. The enzyme was resolved from other protein kinase activities by ion exchange chromatography. 5. Phosphatidylserine and Ca2+ were required for protein kinase C to be active. 6. Diacylglycerol and phorbol ester enhanced the activation of the enzyme.

蛋白激酶C被认为在组织和器官中普遍存在；然而，其分离和特性鉴定主要是在成年哺乳动物组织中进行的。2. 越来越多的证据表明这种酶在早期发育过程中很重要。3. 在本研究中，已在海胆卵的胞质组分中鉴定出蛋白激酶C并对其进行了部分特性分析。4. 通过离子交换色谱法将该酶与其他蛋白激酶活性分离。5. 蛋白激酶C发挥活性需要磷脂酰丝氨酸和Ca2+。6. 二酰基甘油和佛波酯可增强该酶的活性。

Department of Zoology, Iowa State University, Ames 50011.

Comp Biochem Physiol B. 1989;92(2):251-4. doi: 10.1016/0305-0491(89)90274-5.

Protein kinase C is considered to be ubiquitous in tissues and organs; however, its isolation and characterization have been principally with adult mammalian tissues. 2. There is increasing evidence for the importance of this enzyme during early development. 3. In this study, protein kinase C has been identified and partially characterized in cytosolic fraction from sea urchin eggs. 4. The enzyme was resolved from other protein kinase activities by ion exchange chromatography. 5. Phosphatidylserine and Ca2+ were required for protein kinase C to be active. 6. Diacylglycerol and phorbol ester enhanced the activation of the enzyme.

蛋白激酶C被认为在组织和器官中普遍存在；然而，其分离和特性鉴定主要是在成年哺乳动物组织中进行的。2. 越来越多的证据表明这种酶在早期发育过程中很重要。3. 在本研究中，已在海胆卵的胞质组分中鉴定出蛋白激酶C并对其进行了部分特性分析。4. 通过离子交换色谱法将该酶与其他蛋白激酶活性分离。5. 蛋白激酶C发挥活性需要磷脂酰丝氨酸和Ca2+。6. 二酰基甘油和佛波酯可增强该酶的活性。