与登革热病毒相关的流行病毒感染的流行病学因素以及印度东部血清型特异性登革热病毒循环的分子特征。
Episodes of the epidemiological factors correlated with prevailing viral infections with dengue virus and molecular characterization of serotype-specific dengue virus circulation in eastern India.
机构信息
Department of Biotechnology, Sai Nath University, Ranchi, Jharkhand, India.
Central Research Laboratory, Institute of Medical Sciences & Sum Hospital, Siksha 'O' Anusandhan University, K-8, Kalinga Nagar, Bhubaneswar 751003, Odisha, India.
出版信息
Infect Genet Evol. 2018 Mar;58:40-49. doi: 10.1016/j.meegid.2017.12.005. Epub 2017 Dec 13.
BACKGROUND
Dengue is one of the most important and widespread viral infection comprises 4 related serotypes (DEN-1, 2, 3, and 4). Infection with one serotype does not protect against the others, and sequential infections put people at greater risk for dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). This study determines the epidemiology of prevailing viral infections with dengue and molecular characterization of serotype-specific DENV circulation in Odisha of eastern India.
METHODS
During the year 2013, 1980 blood samples with suspected dengue cases were obtained between days 1-10 of illness and analyzed by NS1 Ag-RDT, NS1 Ag-ELISA, and RT-PCR. The differential detection of dengue infections and DENV serotyping were carried out by IgM/IgG Ab-ELISA and RT-PCR, respectively.
RESULTS
Of the 1980 samples, 733 (37.0%) were positive for dengue RNA by RT-PCR. The confirmed cases of dengue were more in males (73.6%) in comparing to females (26.4%). The age group of 15-44years (527 cases, 71.9%) were more susceptible to dengue infections. 656 (89.5%) cases had infected with monotypic infection by different DENV serotype and 77 (10.5%) cases had multitypic infections by multiple serotypes of DENV. Of the total multitypic infections, there were 74 (10.1%) cases had infected with DENV-2 and DENV-3 serotypes at a time; and only 3 (0.4%) cases had the concurrent infections of all three serotypes that were, DENV-1, DENV-2, and DENV-3. Of the 28 DHF cases, there were 17 (2.3%) cases had infected with multitypic infections and 11 (1.5%) cases had infected with monotypic infection.
CONCLUSION
Dengue infections have prevailed from the month of July and grasped it's the peak in September. Rain, temperature and relative humidity have favored the dengue infections. Young adults and males are more susceptible to dengue infections. Serotypes DEN-2 followed by DEN-3 was dominant among the confirmed dengue cases. Co-circulation of multitypic infections with multiple DENV serotypes and the emergence of DHF cases suggested that eastern Indian state Odisha was becoming a hyper-endemic province for dengue; therefore, continuous surveillance is suggested for understanding the epidemiology of the diseases and monitoring the changes in the characteristics of circulating DENV strains.
背景
登革热是最重要和分布最广的病毒感染之一，包括 4 种相关血清型（DEN-1、2、3 和 4）。感染一种血清型并不能预防其他血清型，连续感染会使人更容易患登革出血热（DHF）和登革休克综合征（DSS）。本研究旨在确定印度东部奥里萨邦登革热病毒流行感染的流行病学情况，并对血清型特异性 DENV 循环进行分子特征分析。
方法
在 2013 年期间，采集了 1980 份疑似登革热病例的血液样本，在发病第 1-10 天进行检测，采用 NS1 Ag-RDT、NS1 Ag-ELISA 和 RT-PCR 进行分析。通过 IgM/IgG Ab-ELISA 和 RT-PCR 分别对登革热感染的差异检测和 DENV 血清分型进行。
结果
1980 份样本中，有 733 份（37.0%）通过 RT-PCR 检测到登革热 RNA 呈阳性。与女性（26.4%）相比，男性（73.6%）确诊的登革热病例更多。15-44 岁年龄组（527 例，71.9%）更容易感染登革热。656 例（89.5%）为单型感染，不同的 DENV 血清型，77 例（10.5%）为多型感染，有多个 DENV 血清型。在总多型感染中，有 74 例（10.1%）同时感染了 DENV-2 和 DENV-3 血清型，仅有 3 例（0.4%）同时感染了所有三种血清型，即 DENV-1、DENV-2 和 DENV-3。在 28 例 DHF 病例中，有 17 例（2.3%）为多型感染，11 例（1.5%）为单型感染。
结论
登革热感染从 7 月开始流行，并在 9 月达到高峰。降雨、温度和相对湿度有利于登革热感染。年轻人和男性更容易感染登革热。DEN-2 血清型紧随 DEN-3 血清型，是确诊登革热病例中的主要血清型。多型感染和多血清型 DENV 的同时流行以及 DHF 病例的出现表明，印度东部奥里萨邦正成为登革热的高度流行省份；因此，建议进行持续监测，以了解疾病的流行病学情况，并监测循环 DENV 株特征的变化。
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