电化学检测癌胚抗原。
Electrochemical detection of carcinoembryonic antigen.
机构信息
School of Chemistry and Chemical Engineering, Nantong University, Nantong 226007, PR China; Department of Physical and Environmental Science, University of Toronto Scarborough, 1265 Military Trail, Toronto, Ontario, Canada M1C 1A4.
Department of Physical and Environmental Science, University of Toronto Scarborough, 1265 Military Trail, Toronto, Ontario, Canada M1C 1A4.
出版信息
Biosens Bioelectron. 2018 Apr 15;102:610-616. doi: 10.1016/j.bios.2017.12.014. Epub 2017 Dec 8.
In this work, a sandwich-type electrochemical immunosensor for carcinoembryonic antigen (CEA) detection has been constructed and tested. Unlike many other sensors using external electrochemical species in the electrolyte to generate an electrochemical signal, a ferrocene derivative has been integrated into the design of the sensor to provide an internal reporting system, allowing detection of CEA in buffers and biological samples. Gold nanoparticles, which have been used to increase the conductivity of sensing surfaces, also carry immobilized secondary anti-CEA and a ferrocene derivative. The shelf life testing of the sensor shows good performance after storage for 4 weeks. The sensor has been calibrated against different concentration of the target protein using square wave voltammetry. The calibration curve has been obtained in the range of 0.05-20ngmL, and the detection limit for CEA is ~ 0.01ngmL. The capability of the immunosensor has been verified by performing detection of CEA in human serum samples.
本工作构建并测试了用于癌胚抗原（CEA）检测的三明治型电化学免疫传感器。与许多其他使用电解质中外部电化学物质来产生电化学信号的传感器不同，本传感器的设计中集成了一种二茂铁衍生物，提供了内部报告系统，可用于缓冲液和生物样品中的 CEA 检测。金纳米粒子被用于提高传感表面的导电性，同时也携带固定化的二级抗 CEA 和二茂铁衍生物。传感器的货架寿命测试显示，在储存 4 周后仍具有良好的性能。使用方波伏安法对传感器进行了针对不同浓度目标蛋白的校准。在 0.05-20ngmL 的范围内获得了校准曲线，CEA 的检测限约为 0.01ngmL。通过在人血清样本中进行 CEA 的检测，验证了免疫传感器的性能。
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