Kondoh N, Oikawa T, Yuhki Y, Ogiso Y, Satoh C, Kuzumaki N
Laboratory of Molecular Genetics, Hokkaido University School of Medicine, Japan.
Exp Cell Res. 1989 Apr;181(2):579-83. doi: 10.1016/0014-4827(89)90114-6.
In mouse plasmacytoma (MPC) S194, the rearranged c-myc gene was much more sensitive to DNase I digestion than the nonrearranged gene. The sensitivity of the rearranged c-myc was markedly reduced to the same extent as that of the nonrearranged one in hybrids between the MPC cells and the fibroblasts, but not in a hybrid between the MPC and the spleen cells. These results suggest that trans-acting factors in fibroblasts alter the DNase I-sensitive structure of the rearranged c-myc gene.
在小鼠浆细胞瘤（MPC）S194中，重排的c-myc基因比未重排的基因对DNA酶I消化更敏感。在MPC细胞与成纤维细胞的杂交体中，重排的c-myc的敏感性显著降低至与未重排基因相同的程度，但在MPC与脾细胞的杂交体中则不然。这些结果表明，成纤维细胞中的反式作用因子改变了重排的c-myc基因的DNA酶I敏感结构。
