Russell P, Garland D, Epstein D L
National Eye Institute, National Institutes of Health, Bethesda, MD 20892.
Exp Eye Res. 1989 Feb;48(2):251-60. doi: 10.1016/s0014-4835(89)80074-0.
The proteins from the trabecular meshwork (TM) of calf and cow eyes were analysed to determine if differences in composition were present and to examine whether this tissue could be used as a framework for the study of glaucoma. Differences in polypeptide composition or amount of protein could be detected with extractions using either acetic acid, neutral buffers and urea, or guanidine hydrochloride. In general, the results suggest that an aggregation of proteins may be occurring with aging. The acid-soluble fraction of both calf and cow TM resembled older human TM specimens with the most prominent protein around 68 kD. To test the utility of the bovine TM system, a mixed function oxidation system was used to determine how the proteins of the TM would react to oxidative stress. Aggregation of the proteins in calf TM as well as actin could be demonstrated, consistent with the idea that the aggregation seen in the cow TM might be the result of oxidation of this tissue. The present study lays a foundation for future work on bovine TM and is consistent with the hypothesis that aging changes in this tissue might be a result of oxidative processes.
对小牛和母牛眼睛小梁网（TM）中的蛋白质进行分析，以确定其组成是否存在差异，并研究该组织是否可用作青光眼研究的框架。使用乙酸、中性缓冲液和尿素或盐酸胍进行提取时，可检测到多肽组成或蛋白质含量的差异。总体而言，结果表明蛋白质聚集可能随衰老而发生。小牛和母牛TM的酸溶性部分类似于老年人类TM标本，最突出的蛋白质约为68 kD。为了测试牛TM系统的实用性，使用混合功能氧化系统来确定TM中的蛋白质如何应对氧化应激。可以证明小牛TM中的蛋白质以及肌动蛋白发生了聚集，这与牛TM中所见的聚集可能是该组织氧化结果的观点一致。本研究为未来关于牛TM的研究奠定了基础，并且与该组织衰老变化可能是氧化过程结果的假设一致。
