Department of Food Technology, Engineering and Nutrition, Lund University, Lund, Sweden.
Department of Food Technology, Engineering and Nutrition, Lund University, Lund, Sweden.
J Chromatogr A. 2018 Jan 19;1533:155-163. doi: 10.1016/j.chroma.2017.12.026. Epub 2017 Dec 12.
This study suggests a novel method for determination of the channel height in asymmetrical flow field-flow fractionation (AF4), which can be used for calibration of the channel for hydrodynamic radius determinations. The novel method uses an oil-in-water nanoemulsion together with multi angle light scattering (MALS) and elution theory to determine channel height from an AF4 experiment. The method is validated using two orthogonal methods; first, by using standard particle elution experiments and, secondly, by imaging an assembled and carrier liquid filled channel by x-ray computed tomography (XCT). It is concluded that the channel height can be determined with approximately the same accuracy as with the traditional channel height determination technique. However, the nanoemulsion method can be used under more challenging conditions than standard particles, as the nanoemulsion remains stable in a wider pH range than the previously used standard particles. Moreover, the novel method is also more cost effective.
本研究提出了一种用于测定非对称流场流分离（AF4）中通道高度的新方法，该方法可用于通道的校准，以进行流体力学半径的测定。该新方法使用油包水纳米乳液结合多角度光散射（MALS）和洗脱理论，从 AF4 实验中确定通道高度。该方法通过两种正交方法进行验证；首先，通过使用标准粒子洗脱实验，其次，通过 X 射线计算机断层扫描（XCT）对组装好的载液填充通道进行成像。结论是，与传统的通道高度确定技术相比，该通道高度可以以大致相同的精度确定。然而，纳米乳液方法比标准粒子更适用于更具挑战性的条件，因为纳米乳液在比以前使用的标准粒子更宽的 pH 范围内保持稳定。此外，该新方法还具有更高的成本效益。
