Craig A D, Linington A J, Kniffki K D
Division of Neurobiology, Barrow Neurological Institute, Phoenix, AZ 85013.
Exp Brain Res. 1989;74(2):431-6. doi: 10.1007/BF00248879.
The laminar distributions of spinothalamic tract cells retrogradely labeled by the fluorescent tracers Fast Blue and Diamidino Yellow and by free or lectin-coupled horseradish peroxidase have been found to be significantly different. The total numbers of cells labeled by each method are similar, but nearly twice as many lamina I cells are labeled by the fluorescent tracers and more lamina V cells are labeled by peroxidase. Injection site spread and spurious labeling due to leakage or fibers of passage do not account for these differences. These results indicate that both horseradish peroxidase and fluorescent tracers may be selectively transported and, thus, that the cautious use of both methods should be recommended for analyses of afferent populations.
已发现，用荧光示踪剂快蓝和双脒基黄以及游离或凝集素偶联辣根过氧化物酶逆行标记的脊髓丘脑束细胞的层状分布存在显著差异。每种方法标记的细胞总数相似，但荧光示踪剂标记的I层细胞数量几乎是其两倍，而过氧化物酶标记的V层细胞更多。注射部位扩散以及由于渗漏或通过纤维导致的假标记并不能解释这些差异。这些结果表明，辣根过氧化物酶和荧光示踪剂都可能被选择性转运，因此，对于传入群体的分析，建议谨慎使用这两种方法。
