Grant D M, Lottspeich F, Meyer U A
Department of Pharmacology, University of Basel, Switzerland.
FEBS Lett. 1989 Feb 13;244(1):203-7. doi: 10.1016/0014-5793(89)81193-7.
Acetyl CoA-dependent arylamine N-acetyltransferase (EC 2.3.1.5) is the target of a genetic polymorphism in the metabolism of drugs and carcinogens. N-Acetyltransferase was purified 1000-fold from cytosol of human liver and its identity was verified by amino acid sequence homology of two of its tryptic peptides with published rabbit and chicken N-acetyltransferase sequences. Enzyme activity correlated with the presence of two proteins, NAT-1 and NAT-2, with indistinguishable molecular masses (31 kDa). NAT-1 and NAT-2 could be separated by anion-exchange chromatography and were functionally distinguished by their different apparent affinities for the acceptor amine sulfamethazine (SMZ). Antibodies raised against NAT-1 were able to recognize both isozymes on Western blots.
乙酰辅酶A依赖性芳胺N - 乙酰基转移酶（EC 2.3.1.5）是药物和致癌物代谢中一种遗传多态性的靶点。N - 乙酰基转移酶从人肝细胞溶质中纯化了1000倍，通过其两条胰蛋白酶肽段的氨基酸序列与已发表的兔和鸡N - 乙酰基转移酶序列的同源性验证了其身份。酶活性与两种蛋白质NAT - 1和NAT - 2的存在相关，它们的分子量无法区分（31 kDa）。NAT - 1和NAT - 2可以通过阴离子交换色谱法分离，并且在功能上通过它们对受体胺磺胺二甲嘧啶（SMZ）的不同表观亲和力来区分。针对NAT - 1产生的抗体能够在蛋白质免疫印迹上识别两种同工酶。
