基质金属蛋白酶 1：通过多重微阵列分析，鳞状细胞癌更好的生物标志物。
Matrix metalloproteinase 1: a better biomarker for squamous cell carcinoma by multiple microarray analyses.
机构信息
Department of Dermatology, Chinese PLA General Hospital, Beijing, China.
Department of Dermatology, the 251st Hospital of Chinese PLA, Zhangjiakou, China.
出版信息
G Ital Dermatol Venereol. 2019 Jun;154(3):327-337. doi: 10.23736/S0392-0488.17.05770-4. Epub 2017 Dec 15.
BACKGROUND
The present study aimed to validate MMP1 role in the development of squamous cell carcinoma (SCC) by bioinformatics methods.
METHODS
Gene expression data of 10 GSE series (5 HNSCCs and 5 cSCCs) were obtained from the Gene Expression Omnibus (GEO) database and used to identify differentially expressed genes (DEGs).
RESULTS
Higher expression of MMP1 was found rank number one in 9/10 GSE series of SCC. MMP1 was mainly focused on Gene Ontology (GO) terms of collagen catabolic process, extracellular matrix disassembly. The analysis results of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways mainly involved Rheumatoid arthritis, Bladder cancer and Pathways in cancer. Also, MMP1 was identified as a hub protein in the PPI network by using Cytoscape software. In addition, others MMPs members of family were analyzed.
CONCLUSIONS
These results suggested that MMP1 may be pivotal to the transition from normal skin to premalignant lesions to SCC, thus representing a potential therapeutic target gene of diagnosis and prevention in SCC.
背景
本研究旨在通过生物信息学方法验证 MMP1 在鳞状细胞癌 (SCC) 发展中的作用。
方法
从基因表达综合数据库 (GEO) 中获取了 10 个 GSE 系列 (5 个 HNSCC 和 5 个 cSCC) 的基因表达数据，用于鉴定差异表达基因 (DEGs)。
结果
在 10 个 SCC 的 GSE 系列中，有 9 个发现 MMP1 的表达水平排名第一。MMP1 主要集中在胶原分解代谢、细胞外基质解体等基因本体论 (GO) 术语上。京都基因与基因组百科全书 (KEGG) 通路的分析结果主要涉及类风湿性关节炎、膀胱癌和癌症通路。此外，还使用 Cytoscape 软件确定了 PPI 网络中的 MMP1 为枢纽蛋白。此外，还分析了其他 MMP 家族成员。
结论
这些结果表明，MMP1 可能在正常皮肤向癌前病变到 SCC 的转变中起着关键作用，因此代表了 SCC 诊断和预防的潜在治疗靶基因。
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