Isolation and reconstitution of the high-affinity choline carrier.
作者信息
Knipper M, Boekhoff I, Breer H
机构信息
University Stutgart-Hohenheim, Institute for Zoophysiology, FRG.
出版信息
FEBS Lett. 1989 Mar 13;245(1-2):235-7. doi: 10.1016/0014-5793(89)80228-5.
PMID:2924923
Abstract
Monoclonal antibodies, which block the high-affinity uptake of choline in synaptosomal ghosts, have been used to purify a membrane polypeptide (80 kDa) from insect synaptosomal membranes. This isolated protein was found to catalyse the sodium-dependent, hemicholinium-sensitive accumulation of choline after reconstitution into liposomes, thus, apparently represents the high-affinity choline transporter.
摘要
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