Du Zhaojin, Qiu Zhilei, Wang Zhankun, Wang Xinsheng
Reproductive Medical Center, Qingdao Women and Children's Hospital, Qingdao University, Qingdao, Shandong 266034, P.R. China.
Department of Urology, Qingdao Municipal Hospital, Qingdao, Shandong 266071, P.R. China.
Exp Ther Med. 2018 Jan;15(1):305-309. doi: 10.3892/etm.2017.5359. Epub 2017 Oct 24.
The aim of the study was to investigate the inhibitory effects of soybean isoflavones (SI) on testicular cell apoptosis in mice with type-2 diabetes, as well as any possible mechanisms of action. Thirty male C57BL/6J mice were randomly divided into the control, diabetic (model), and treatment (SI) groups (n=10 each). After treatment for 20 weeks, testicular cell apoptosis was detected and evaluated using DAPI staining. The expression and distribution of caspase-3 protein in testicular tissues was detected via immunohistochemistry, while caspase-3 mRNA expression was detected using RT-PCR. Bax and Bcl-2 protein expression was detected by western blot analysis. At week 20, DAPI staining showed that SI treatment significantly decreased testicular tissue cell apoptosis in diabetic mice. Immunohistochemical staining revealed that caspase-3 expression in the SI group was significantly reduced relative to the model group. RT-PCR showed that SI treatment significantly decreased caspase-3 mRNA expression relative to the model group. Western blot analysis revealed that SI treatment significantly decreased Bax protein expression and increased Bcl-2 protein expression (P<0.01). SI exhibited an inhibitory effect on testicular tissue cell apoptosis in mice with type 2 diabetes, with this effect possibly mediated by a decreased expression of caspase-3 and Bax and increased Bcl-2 protein expression.
本研究旨在探讨大豆异黄酮（SI）对2型糖尿病小鼠睾丸细胞凋亡的抑制作用及其可能的作用机制。将30只雄性C57BL/6J小鼠随机分为对照组、糖尿病（模型）组和治疗（SI）组（每组n = 10）。治疗20周后，采用DAPI染色检测并评估睾丸细胞凋亡情况。通过免疫组织化学检测睾丸组织中caspase-3蛋白的表达和分布，同时采用RT-PCR检测caspase-3 mRNA表达。通过蛋白质印迹分析检测Bax和Bcl-2蛋白表达。在第20周时，DAPI染色显示，SI治疗显著降低了糖尿病小鼠睾丸组织细胞凋亡。免疫组织化学染色显示，与模型组相比，SI组中caspase-3表达显著降低。RT-PCR显示，与模型组相比，SI治疗显著降低了caspase-3 mRNA表达。蛋白质印迹分析显示，SI治疗显著降低了Bax蛋白表达并增加了Bcl-2蛋白表达（P<0.01）。SI对2型糖尿病小鼠睾丸组织细胞凋亡具有抑制作用，其作用可能是通过降低caspase-3和Bax的表达以及增加Bcl-2蛋白表达来介导的。
