Copeland P A, Thomas P
University of Texas at Austin, Marine Science Institute, Port Aransas 78373-1267.
Gen Comp Endocrinol. 1989 Mar;73(3):425-41. doi: 10.1016/0016-6480(89)90200-1.
The isolation of a maturational gonadotropin from pituitaries of Atlantic croaker (Micropogonias undulatus), a marine teleost belonging to the order perciformes, is described. A radioimmunoassay has been developed for the measurement of this hormone in blood samples and validation of the assay is presented. The hormone measured is an acidic isoform of GTH and can be detected in the plasma at concentrations from 25 to 0.05 ng/ml. At least one other gonadotropic fraction was detected during the purification. The acidic form of GTH is released from croaker pituitaries after stimulation with luteinizing hormone-releasing hormone analog (LHRHa), both in vivo and in vitro. The GTH is steroidogenic, is active in an in vitro oocyte germinal-vesicle breakdown assay, and is elevated in the blood of fish undergoing LHRHa-induced ovulation.
本文描述了从鲈形目硬骨鱼大西洋鲳（Micropogonias undulatus）的垂体中分离出一种成熟促性腺激素的过程。已开发出一种放射免疫分析法用于测量血液样本中的这种激素，并给出了该分析方法的验证情况。所测量的激素是促性腺激素（GTH）的一种酸性异构体，在血浆中的检测浓度范围为25至0.05 ng/ml。在纯化过程中至少检测到了一种其他的促性腺激素组分。无论是在体内还是体外，用促黄体激素释放激素类似物（LHRHa）刺激后，大西洋鲳垂体都会释放出酸性形式的GTH。这种GTH具有类固醇生成作用，在体外卵母细胞生发泡破裂试验中具有活性，并且在接受LHRHa诱导排卵的鱼的血液中含量会升高。
