Huang Ping, Xin Wenxiu, Zheng Xiaowei, Luo Fang, Li Qinglin, Lv Guiyuan
1 College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, PR China.
2 Department of Pharmacy, Zhejiang Cancer Hospital, Hangzhou, PR China.
J Int Med Res. 2017 Dec;45(6):1985-2000. doi: 10.1177/0300060517722876. Epub 2017 Sep 27.
Objectives Sceptridium ternatum is an expectorant in traditional Chinese medicine and is prescribed for the treatment of asthma. The study aim was to screen Sceptridium ternatum for ingredients with antitussive and antiasthmatic effects and to study their associated mechanisms. Methods Cough in mice was induced using ammonia. Cough latency and the number of coughs within 3 minutes were determined. Airway responsiveness was assessed using ovalbumin as a sensitizer and characteristic asthma indicators were measured. Results Chloroform and ethyl acetate extracts significantly reduced the number of coughs within 3 minutes, tidal volume, and the percentage of eosinophilic granulocytes, lymphocytes and neutrophils. All extracts decreased airway responsiveness in asthmatic mice compared with the untreated group. Petroleum ether, chloroform and n-butanol extracts lowered the Penh values of asthmatic mice. Petroleum ether and ethyl acetate extracts greatly reduced interleukin-4 expression and the interleukin-4/interferon gamma ratio. Compared with the model group, all extracts reduced mRNA expression of the cysteinyl leukotriene receptor-1 (CysLT). Conclusions Chloroform extract and ethyl acetate extract displayed obvious antitussive effects and reduced airway inflammation. Thus, these two extracts contain the effective ingredients of Sceptridium ternatum. The active mechanism was ascribed to inhibition of mRNA expression of the CysLT receptor in mice with bronchial asthma.
目的 阴地蕨是一种传统中药中的祛痰剂，常用于治疗哮喘。本研究旨在筛选阴地蕨中具有镇咳和平喘作用的成分，并研究其相关机制。方法 用氨水诱导小鼠咳嗽，测定咳嗽潜伏期和3分钟内的咳嗽次数。以卵清蛋白作为致敏剂评估气道反应性，并测量特征性哮喘指标。结果 氯仿提取物和乙酸乙酯提取物显著减少了3分钟内的咳嗽次数、潮气量以及嗜酸性粒细胞、淋巴细胞和中性粒细胞的百分比。与未处理组相比，所有提取物均降低了哮喘小鼠的气道反应性。石油醚、氯仿和正丁醇提取物降低了哮喘小鼠的Penh值。石油醚提取物和乙酸乙酯提取物大大降低了白细胞介素-4的表达以及白细胞介素-4/干扰素γ的比值。与模型组相比，所有提取物均降低了半胱氨酰白三烯受体-1（CysLT）的mRNA表达。结论 氯仿提取物和乙酸乙酯提取物具有明显的镇咳作用，并减轻了气道炎症。因此，这两种提取物含有阴地蕨的有效成分。其作用机制归因于对支气管哮喘小鼠CysLT受体mRNA表达的抑制。
