Ferrari-Dileo G, Davis E B, Anderson D R
Bascom Palmer Eye Institute, University of Miami School of Medicine, Department of Ophthalmology, FL 33101.
Invest Ophthalmol Vis Sci. 1989 Mar;30(3):473-7.
Blood vessels from human, cat, pig and bovine retinas were analyzed for their contents of choline acetyltransferase (ChAT) and muscarinic binding sites. ChAT was measured by the synthesis of 3H-acetylcholine in the presence of 3H-acetyl CoA and choline. Muscarinic binding sites were determined by the specific binding of 3H-quinuclidinyl benzylate (3H-QNB). Tissue levels of ChAT varied from 39-850 nmol/g/hr, the lowest values being in human tissues. Muscarinic binding sites were less different (0.5 to 1.8 fmol/mg net weight) among the species studied, being the highest values in human retinal vessels. It appears that retinal blood vessels not only have sites to bind acetylcholine, perhaps to mediate physiologic responses, but might be capable of supplying acetylcholine for local vascular tone control.
对来自人类、猫、猪和牛视网膜的血管进行了分析，以检测其胆碱乙酰转移酶（ChAT）含量和毒蕈碱结合位点。ChAT通过在3H-乙酰辅酶A和胆碱存在的情况下合成3H-乙酰胆碱来测定。毒蕈碱结合位点通过3H-喹核醇基苯甲酸酯（3H-QNB）的特异性结合来确定。ChAT的组织水平在39 - 850 nmol/g/小时之间变化，最低值出现在人类组织中。在所研究的物种中，毒蕈碱结合位点的差异较小（0.5至1.8 fmol/mg净重），在人类视网膜血管中的值最高。看来视网膜血管不仅有结合乙酰胆碱的位点，可能用于介导生理反应，而且可能能够提供乙酰胆碱以控制局部血管张力。
