纤维连接蛋白与 SsPepO 的结合促进猪链球菌脑膜炎的发展。
Binding of Fibronectin to SsPepO Facilitates the Development of Streptococcus suis Meningitis.
机构信息
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, China.
出版信息
J Infect Dis. 2018 Mar 5;217(6):973-982. doi: 10.1093/infdis/jix523.
BACKGROUND
SsPepO is an important virulence in Streptococcus suis.
METHODS
In this study, we showed that SsPepO contributes to the human fibronectin-mediated adherence ability of S. suis to human brain microvascular endothelial cells.
RESULTS
The addition of an antifibronectin antibody or an arginine-glycine-aspartic acid peptide that blocks fibronectin binding to integrins significantly reduced adherence of the wild-type but not the SspepO mutant strain, indicating the importance of the SsPepO-fibronectin-integrin interaction for S. suis cellular adherence.
CONCLUSIONS
By analyzing Evans blue extravasation in vivo, we showed that the interaction between SsPepO and human fibronectin significantly increased permeability of the blood-brain barrier. Furthermore, the SspepO mutant caused lower bacterial loads in the brain than wild-type S. suis in models of meningitis. These data demonstrate that SsPepO is a fibronectin-binding protein, which plays a contributing role in the development of S. suis meningitis.
背景
SsPepO 是猪链球菌的一种重要毒力因子。
方法
本研究表明 SsPepO 有助于猪链球菌与人纤维连接蛋白介导的黏附能力，从而黏附于人脑微血管内皮细胞。
结果
添加抗纤维连接蛋白抗体或精氨酸-甘氨酸-天冬氨酸肽（可阻断纤维连接蛋白与整合素结合）可显著降低野生型但不降低 SspepO 突变株的黏附率，表明 SsPepO-纤维连接蛋白-整合素相互作用对猪链球菌细胞黏附的重要性。
结论
通过分析体内 Evans 蓝渗出，我们表明 SsPepO 与人纤维连接蛋白的相互作用显著增加了血脑屏障的通透性。此外，脑膜炎模型中 SspepO 突变株引起的脑部细菌载量低于野生型猪链球菌。这些数据表明 SsPepO 是一种纤维连接蛋白结合蛋白，它在猪链球菌脑膜炎的发展中起作用。
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