State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, China.
J Infect Dis. 2018 Mar 5;217(6):973-982. doi: 10.1093/infdis/jix523.
SsPepO is an important virulence in Streptococcus suis.
In this study, we showed that SsPepO contributes to the human fibronectin-mediated adherence ability of S. suis to human brain microvascular endothelial cells.
The addition of an antifibronectin antibody or an arginine-glycine-aspartic acid peptide that blocks fibronectin binding to integrins significantly reduced adherence of the wild-type but not the SspepO mutant strain, indicating the importance of the SsPepO-fibronectin-integrin interaction for S. suis cellular adherence.
By analyzing Evans blue extravasation in vivo, we showed that the interaction between SsPepO and human fibronectin significantly increased permeability of the blood-brain barrier. Furthermore, the SspepO mutant caused lower bacterial loads in the brain than wild-type S. suis in models of meningitis. These data demonstrate that SsPepO is a fibronectin-binding protein, which plays a contributing role in the development of S. suis meningitis.
SsPepO 是猪链球菌的一种重要毒力因子。
本研究表明 SsPepO 有助于猪链球菌与人纤维连接蛋白介导的黏附能力，从而黏附于人脑微血管内皮细胞。
添加抗纤维连接蛋白抗体或精氨酸-甘氨酸-天冬氨酸肽（可阻断纤维连接蛋白与整合素结合）可显著降低野生型但不降低 SspepO 突变株的黏附率，表明 SsPepO-纤维连接蛋白-整合素相互作用对猪链球菌细胞黏附的重要性。
通过分析体内 Evans 蓝渗出，我们表明 SsPepO 与人纤维连接蛋白的相互作用显著增加了血脑屏障的通透性。此外，脑膜炎模型中 SspepO 突变株引起的脑部细菌载量低于野生型猪链球菌。这些数据表明 SsPepO 是一种纤维连接蛋白结合蛋白，它在猪链球菌脑膜炎的发展中起作用。
