Di Spigna G, Iannone M, Ladogana P, Salzano S, Ventre M, Covelli B, De Marinis E, Postiglione L
Department of Translational Medical Sciences, University of Naples Federico II, Naples, Italy.
Interdisciplinary Research Centre on Biomaterials. Department of Chemical, Materials and Industrial Production Engineering, University of Naples Federico II, Italy.
J Biol Regul Homeost Agents. 2017 Oct-Dec;31(4):911-921.
Myocardial infarction is the leading cause of morbidity and mortality in developed countries. It causes a left ventricular dysfunction, mainly due to the loss of functional tissue, resulting in heart failure. New therapies are being developed, using a tissue engineering approach, with the ultimate goal of restoring cardiac function by regenerating and repairing the damaged myocardium. In the present study we investigated the behaviour of a specific population of c-kit positive human cardiac stem cells, called Multipotent Adult Stem Cells (MASCs), grown within three-dimensional collagen scaffolds (3D), to establish whether they could be used in post-infarction cardiac regeneration. We also evaluated the expression levels of the Granulocyte Macrophage-Colony Stimulating Factor Receptor (GM-CSFR) and endoglin, a component of the Transforming Growth Factor beta (TGF-ß) receptor complex. Finally, we also evaluated the expression of the α2β1integrin. MASCs cultured within 3D collagen matrices are able to proliferate and migrate even in the absence of chemotactic agents and express high levels of factors involved in cell proliferation and migration, such as GM-CSFRα chain and integrins. They therefore represent a promising approach to tissue engineering aimed to restore cardiac function. Our results also suggest a role of GM-CSF in cell proliferation, while TGF-β does not seem to be relevant.
心肌梗死是发达国家发病和死亡的主要原因。它会导致左心室功能障碍，主要是由于功能性组织的丧失，进而引发心力衰竭。目前正在采用组织工程方法开发新的治疗方法，其最终目标是通过再生和修复受损心肌来恢复心脏功能。在本研究中，我们调查了一种特定的c-kit阳性人类心脏干细胞群体，即多能成体干细胞（MASC），在三维胶原支架（3D）内生长的行为，以确定它们是否可用于心肌梗死后的心脏再生。我们还评估了粒细胞巨噬细胞集落刺激因子受体（GM-CSFR）和内皮糖蛋白（一种转化生长因子β（TGF-β）受体复合物的成分）的表达水平。最后，我们还评估了α2β1整合素的表达。在3D胶原基质中培养的MASC即使在没有趋化剂的情况下也能够增殖和迁移，并表达高水平的参与细胞增殖和迁移的因子，如GM-CSFRα链和整合素。因此，它们代表了一种有前景的组织工程方法，旨在恢复心脏功能。我们的结果还表明GM-CSF在细胞增殖中起作用，而TGF-β似乎无关。
