• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

稻米籽粒锰积累主效 QTL 的鉴定。

Characterization of a major QTL for manganese accumulation in rice grain.

机构信息

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China.

出版信息

Sci Rep. 2017 Dec 18;7(1):17704. doi: 10.1038/s41598-017-18090-7.

DOI:10.1038/s41598-017-18090-7
PMID:29255144
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5735179/
Abstract

Some diets lack sufficient manganese (Mn), an essential mineral. Increasing Mn in grain by biofortification could prevent Mn deficiency, but may increase levels of the toxic element cadmium (Cd). Here, we investigated Mn in rice (Oryza sativa) grains in recombinant inbred lines (RILs) from the cross of 93-11 (low grain Mn) with PA64s (high grain Mn). Quantitative trait locus (QTL) analysis to identify loci controlling grain Mn identified a major QTL, qGMN7.1, on the short arm of chromosome 7; qGMN7.1 explained 15.6% and 22.8% of the phenotypic variation in the RIL populations grown in two distinct environments. We validated the QTL with a chromosome segment substitution line (CSSL), CSSL-qGMN7.1, in the 93-11 background harboring qGMN7.1 from PA64s. Compared to 93-11, CSSL-qGMN7.1 grain had increased Mn and decreased Cd concentrations; CSSL-qGMN7.1 roots also showed enhanced Mn uptake. Fine mapping delimited qGMN7.1 to a 49.3-kb region containing OsNRAMP5, a gene responsible for Mn and Cd uptake. Sequence variations in the OsNRAMP5 promoter caused changes in its transcript level, and in grain Mn levels. Our study thus cloned a major QTL for grain Mn concentration in rice, and identified materials for breeding rice for high Mn and low Cd concentrations in the grain.

摘要

一些饮食缺乏足够的锰（Mn），这是一种必需的矿物质。通过生物强化增加谷物中的 Mn 可以预防 Mn 缺乏症，但可能会增加有毒元素镉（Cd）的含量。在这里，我们研究了杂交 93-11（低谷物 Mn）与 PA64s（高谷物 Mn）的重组自交系（RILs）中水稻（Oryza sativa）谷物中的 Mn。用于鉴定控制谷物 Mn 的基因座的数量性状基因座（QTL）分析，鉴定了一个位于第 7 号染色体短臂上的主要 QTL，qGMN7.1；qGMN7.1 解释了在两个不同环境中生长的 RIL 群体中 15.6%和 22.8%的表型变异。我们使用 93-11 背景中的染色体片段替换系（CSSL），CSSL-qGMN7.1，在 PA64s 中含有 qGMN7.1，对 QTL 进行了验证。与 93-11 相比，CSSL-qGMN7.1 谷物的 Mn 浓度增加，Cd 浓度降低；CSSL-qGMN7.1 根系也表现出增强的 Mn 吸收。精细图谱将 qGMN7.1 限定在一个包含 OsNRAMP5 的 49.3-kb 区域内，该基因负责 Mn 和 Cd 的吸收。OsNRAMP5 启动子中的序列变异导致其转录水平和谷物 Mn 水平发生变化。因此，我们克隆了水稻中一个主要的谷物 Mn 浓度 QTL，并鉴定了用于培育高 Mn 和低 Cd 浓度谷物的材料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa3/5735179/769548f9df88/41598_2017_18090_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa3/5735179/6e42ddd9848d/41598_2017_18090_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa3/5735179/647bc814ec31/41598_2017_18090_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa3/5735179/44c0e7828e93/41598_2017_18090_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa3/5735179/dab522250e80/41598_2017_18090_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa3/5735179/a501be1843ef/41598_2017_18090_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa3/5735179/2b0012cbe14c/41598_2017_18090_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa3/5735179/769548f9df88/41598_2017_18090_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa3/5735179/6e42ddd9848d/41598_2017_18090_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa3/5735179/647bc814ec31/41598_2017_18090_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa3/5735179/44c0e7828e93/41598_2017_18090_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa3/5735179/dab522250e80/41598_2017_18090_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa3/5735179/a501be1843ef/41598_2017_18090_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa3/5735179/2b0012cbe14c/41598_2017_18090_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aaa3/5735179/769548f9df88/41598_2017_18090_Fig7_HTML.jpg

相似文献

1
Characterization of a major QTL for manganese accumulation in rice grain.稻米籽粒锰积累主效 QTL 的鉴定。
Sci Rep. 2017 Dec 18;7(1):17704. doi: 10.1038/s41598-017-18090-7.
2
Development of nutritious rice with high zinc/selenium and low cadmium in grains through QTL pyramiding.通过 QTL 聚合技术开发富含锌/硒、低镉的营养型大米。
J Integr Plant Biol. 2020 Mar;62(3):349-359. doi: 10.1111/jipb.12909.
3
A major quantitative trait locus for increasing cadmium-specific concentration in rice grain is located on the short arm of chromosome 7.一个提高水稻籽粒镉特定浓度的主要数量性状位点位于 7 号染色体的短臂上。
J Exp Bot. 2010 Mar;61(3):923-34. doi: 10.1093/jxb/erp360. Epub 2009 Dec 18.
4
Targeted mapping of Cdu1, a major locus regulating grain cadmium concentration in durum wheat (Triticum turgidum L. var durum).靶向定位调控硬粒小麦（Triticum turgidum L. var durum）籽粒镉浓度的主效位点 Cdu1。
Theor Appl Genet. 2010 Oct;121(6):1047-58. doi: 10.1007/s00122-010-1370-1. Epub 2010 Jun 18.
5
Genetic mapping of ionomic quantitative trait loci in rice grain and straw reveals OsMOT1;1 as the putative causal gene for a molybdenum QTL qMo8.在水稻籽粒和秸秆的离子组数量性状位点的遗传定位中发现 OsMOT1;1 是钼 QTL qMo8 的假定因果基因。
Mol Genet Genomics. 2020 Mar;295(2):391-407. doi: 10.1007/s00438-019-01632-1. Epub 2019 Dec 3.
6
Genome wide association mapping for grain shape traits in indica rice.籼稻粒形性状的全基因组关联图谱分析
Planta. 2016 Oct;244(4):819-30. doi: 10.1007/s00425-016-2548-9. Epub 2016 May 19.
7
Quantitative trait loci (QTL) analysis for rice grain width and fine mapping of an identified QTL allele gw-5 in a recombination hotspot region on chromosome 5.水稻粒宽的数量性状位点（QTL）分析及5号染色体上一个重组热点区域中已鉴定的QTL等位基因gw-5的精细定位。
Genetics. 2008 Aug;179(4):2239-52. doi: 10.1534/genetics.108.089862. Epub 2008 Aug 9.
8
Fine mapping of a grain weight quantitative trait locus on rice chromosome 8 using near-isogenic lines derived from a cross between Oryza sativa and Oryza rufipogon.利用源自栽培稻和野生稻杂交后代的近等基因系对水稻第8号染色体上的粒重数量性状位点进行精细定位。
Theor Appl Genet. 2006 Sep;113(5):885-94. doi: 10.1007/s00122-006-0348-5. Epub 2006 Jul 19.
9
Overexpression of the manganese/cadmium transporter OsNRAMP5 reduces cadmium accumulation in rice grain.锰/镉转运蛋白OsNRAMP5的过表达降低了水稻籽粒中的镉积累。
J Exp Bot. 2020 Sep 19;71(18):5705-5715. doi: 10.1093/jxb/eraa287.
10
A weak allele of OsNRAMP5 confers moderate cadmium uptake while avoiding manganese deficiency in rice.水稻中OsNRAMP5的一个弱等位基因赋予适度的镉吸收能力，同时避免锰缺乏。
J Exp Bot. 2022 Oct 18;73(18):6475-6489. doi: 10.1093/jxb/erac302.

引用本文的文献

1
Rice Adaptation to Abiotic Stresses Caused by Soil Inorganic Elements.水稻对土壤无机元素引起的非生物胁迫的适应性
Int J Mol Sci. 2025 Jul 23;26(15):7116. doi: 10.3390/ijms26157116.
2
OsHMA3 overexpression works more efficiently in generating low-Cd rice grain than OsNramp5 knockout mutation.与OsNramp5基因敲除突变相比，过表达OsHMA3在培育低镉水稻籽粒方面效果更佳。
BMC Res Notes. 2025 Feb 5;18(1):55. doi: 10.1186/s13104-025-07112-7.
3
Pedigree genome data of an early-matured Geng/japonica glutinous rice mega variety Longgeng 57.

本文引用的文献

1
Knockout of OsNramp5 using the CRISPR/Cas9 system produces low Cd-accumulating indica rice without compromising yield.利用 CRISPR/Cas9 系统敲除 OsNramp5 可产生低镉积累的籼稻，而不影响产量。
Sci Rep. 2017 Oct 31;7(1):14438. doi: 10.1038/s41598-017-14832-9.
2
Manganese exposure and neurotoxic effects in children.锰暴露与儿童神经毒性效应。
Environ Res. 2017 May;155:380-384. doi: 10.1016/j.envres.2017.03.003. Epub 2017 Mar 10.
3
Regulatory Role of in the Determination of Glumes Fate, Grain Yield, and Quality in Rice.
早熟粳稻超级品种隆粳 57 的系谱基因组数据。
Sci Data. 2024 Feb 22;11(1):230. doi: 10.1038/s41597-024-03057-x.
4
Assessment of Biofortification Approaches Used to Improve Micronutrient-Dense Plants That Are a Sustainable Solution to Combat Hidden Hunger.用于改善富含微量营养素植物的生物强化方法评估，这些植物是应对隐性饥饿的可持续解决方案。
J Soil Sci Plant Nutr. 2022;22(1):475-500. doi: 10.1007/s42729-021-00663-1. Epub 2021 Nov 4.
5
Validating a segment on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety.验证第 7 染色体上的一个片段，以建立低镉积累的籼稻品种。
Sci Rep. 2021 Mar 15;11(1):6053. doi: 10.1038/s41598-021-85324-0.
6
Genome-wide association mapping for grain manganese in rice (Oryza sativa L.) using a multi-experiment approach.利用多实验方法进行水稻（Oryza sativa L.）全基因组关联分析研究谷物锰含量。
Heredity (Edinb). 2021 Mar;126(3):505-520. doi: 10.1038/s41437-020-00390-w. Epub 2020 Nov 24.
7
Mineral and Fatty Acid Content Variation in White Oat Genotypes Grown in Brazil.巴西种植的白燕麦基因型中矿物质和脂肪酸含量的变化
Biol Trace Elem Res. 2021 Mar;199(3):1194-1206. doi: 10.1007/s12011-020-02229-1. Epub 2020 Jun 14.
8
Genetic architecture of subspecies divergence in trace mineral accumulation and elemental correlations in the rice grain.亚种间痕量矿物质积累和稻米元素相关性的遗传结构。
Theor Appl Genet. 2020 Feb;133(2):529-545. doi: 10.1007/s00122-019-03485-z. Epub 2019 Nov 16.
9
Comprehensive analysis of variation of cadmium accumulation in rice and detection of a new weak allele of OsHMA3.水稻镉积累变异的综合分析及 OsHMA3 新弱等位基因的检测。
J Exp Bot. 2019 Nov 18;70(21):6389-6400. doi: 10.1093/jxb/erz400.
10
Mapping QTLs of flag leaf morphological and physiological traits related to aluminum tolerance in wheat ( L.).定位小麦（L.）中与耐铝性相关的旗叶形态和生理性状的数量性状基因座
Physiol Mol Biol Plants. 2019 Jul;25(4):975-990. doi: 10.1007/s12298-019-00670-8. Epub 2019 May 6.
[具体物质]在水稻颖壳命运、籽粒产量和品质决定中的调控作用
Front Plant Sci. 2016 Dec 15;7:1853. doi: 10.3389/fpls.2016.01853. eCollection 2016.
4
Toxic Heavy Metal and Metalloid Accumulation in Crop Plants and Foods.作物植物和食物中的有毒重金属和类金属积累。
Annu Rev Plant Biol. 2016 Apr 29;67:489-512. doi: 10.1146/annurev-arplant-043015-112301. Epub 2016 Jan 21.
5
Genome-wide Association Mapping of Quantitative Trait Loci (QTLs) for Contents of Eight Elements in Brown Rice (Oryza sativa L.).全基因组关联分析糙米中八种元素含量的数量性状基因座（QTLs）。
J Agric Food Chem. 2015 Sep 16;63(36):8008-16. doi: 10.1021/acs.jafc.5b01191. Epub 2015 Sep 8.
6
The OsSPL16-GW7 regulatory module determines grain shape and simultaneously improves rice yield and grain quality.OsSPL16-GW7 调控模块决定粒形，同时提高水稻产量和品质。
Nat Genet. 2015 Aug;47(8):949-54. doi: 10.1038/ng.3352. Epub 2015 Jul 6.
7
Rare allele of a previously unidentified histone H4 acetyltransferase enhances grain weight, yield, and plant biomass in rice.一种先前未鉴定的组蛋白H4乙酰转移酶的稀有等位基因可提高水稻的粒重、产量和植株生物量。
Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):76-81. doi: 10.1073/pnas.1421127112. Epub 2014 Dec 22.
8
OsNRAMP5 contributes to manganese translocation and distribution in rice shoots.OsNRAMP5有助于水稻地上部中锰的转运和分布。
J Exp Bot. 2014 Sep;65(17):4849-61. doi: 10.1093/jxb/eru259. Epub 2014 Jun 24.
9
From laboratory to field: OsNRAMP5-knockdown rice is a promising candidate for Cd phytoremediation in paddy fields.从实验室到田间：敲低OsNRAMP5基因的水稻是稻田镉植物修复的一个有前景的候选品种。
PLoS One. 2014 Jun 5;9(6):e98816. doi: 10.1371/journal.pone.0098816. eCollection 2014.
10
Cadmium transport and tolerance in rice: perspectives for reducing grain cadmium accumulation.水稻中镉的转运与耐受性：降低籽粒镉积累的研究展望
Rice (N Y). 2012 Feb 27;5(1):5. doi: 10.1186/1939-8433-5-5. eCollection 2012.