Murdoch Childrens Research Institute, Parkville, Victoria, 3052, Australia.
Raymond Purves Bone and Joint Research Laboratories, Kolling Institute of Medical Research, Institute of Bone and Joint Research, University of Sydney, St Leonards, New South Wales, 2065, Australia.
Sci Rep. 2017 Dec 18;7(1):17701. doi: 10.1038/s41598-017-17545-1.
To better understand the molecular processes involved in driving osteoarthritis disease progression we characterized expression profiles of microRNAs (miRNA) and mRNAs in synovial tissue from a post-traumatic OA mouse model. OA was induced in 10-12 week old male C57BL6 mice by bilateral surgical destabilization of the medial meniscus (DMM). RNA isolated from the anterior synovium of mice at 1 and 6 weeks post-surgery was subject to expression profiling using Agilent microarrays and qPCR. OA severity was determined histologically. Anterior and posterior synovitis decreased with post-operative time after sham and DMM. No differences in synovitis parameters were evident between sham and DMM in the anterior synovium at either time. While expression profiling revealed 394 miRNAs were dysregulated between 1 and 6 week time-points in the anterior synovium, there were no significant changes in miRNA or mRNA expression between DMM and sham mice at both time-points. Bioinformatic analysis of the miRNAs and mRNAs differentially expressed in tandem with the resolution of anterior synovial inflammation revealed similar biological processes and functions, including organismal injury, connective tissue disorder and inflammatory responses. Our data demonstrates that early OA-specific patterns of synovial miRNAs or mRNAs dysregulation could not be identified in this model of post-traumatic OA.
为了更好地理解导致骨关节炎疾病进展的分子过程，我们对创伤性骨关节炎小鼠模型的滑膜组织中的 microRNAs（miRNA）和 mRNAs 的表达谱进行了特征描述。通过双侧内侧半月板（DMM）手术不稳定，在 10-12 周龄雄性 C57BL6 小鼠中诱导 OA。在手术后 1 周和 6 周，从小鼠的前滑膜中分离 RNA，并用 Agilent 微阵列和 qPCR 进行表达谱分析。OA 严重程度通过组织学确定。手术后时间的增加导致前滑膜的前滑膜炎和后滑膜炎减少。在任何时间，在前滑膜中，假手术和 DMM 之间的滑膜炎参数均无明显差异。虽然表达谱分析显示在前滑膜中，1 周至 6 周时间点之间有 394 个 miRNA 失调，但在两个时间点，DMM 和假手术小鼠之间的 miRNA 或 mRNA 表达均无显着变化。与前滑膜炎症消退相关的 miRNA 和 mRNAs 的差异表达的生物信息学分析揭示了相似的生物学过程和功能，包括机体损伤、结缔组织紊乱和炎症反应。我们的数据表明，在这种创伤性骨关节炎模型中，无法确定早期 OA 特异性滑膜 miRNA 或 mRNAs 失调的模式。
