Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaakob Latif, Bandar Tun Razak, 56000, Cheras, Kuala Lumpur, Malaysia.
Department of Anatomy, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaakob Latif, Bandar Tun Razak, 56000, Cheras, Kuala Lumpur, Malaysia.
Biomed Pharmacother. 2018 Feb;98:191-200. doi: 10.1016/j.biopha.2017.12.042. Epub 2017 Dec 27.
This study aimed to investigate the bone quality in rats induced with metabolic syndrome (MetS) using high-carbohydrate high-fat (HCHF) diet. Male Wistar rats (n = 14) were randomized into two groups. The normal group was given standard rat chow. The MetS group was given HCHF diet. Diet regimen was assigned for a period of 20 weeks. Metabolic syndrome parameters were measured monthly until MetS was established. Left tibiae were scanned using micro-computed tomography at week 0, 8, 12, 16, and 20 to analyze the trabecular and cortical bone structure. At the end of the study, rats were euthanized and their bones were harvested for analysis. Metabolic syndrome was established at week 12 in the HCHF rats. Significant deterioration of trabecular bone was observed at week 20 in the HCHF group (p < 0.05). The HCHF diet also decreased cortical and tissue area significantly (p < 0.05), but did not affect cortical thickness and bone calcium content (p > 0.05). Femur length and width in the HCHF group were significantly shorter than the normal group (p < 0.05). The biomechanical strength test showed that the femur of the HCHF rats could endure significantly lower force, but significantly higher displacement and strain compared to the normal rats (p < 0.05). In conclusion, HCHF diet-induced MetS can cause adverse effects on the bone.
本研究旨在使用高碳水化合物高脂肪（HCHF）饮食诱导代谢综合征（MetS）大鼠，探讨其骨质量。雄性 Wistar 大鼠（n=14）随机分为两组。正常组给予标准大鼠饲料。代谢综合征组给予 HCHF 饮食。饮食方案分配 20 周。每月测量代谢综合征参数，直到建立代谢综合征。在第 0、8、12、16 和 20 周时使用 micro-CT 扫描左胫骨，分析小梁和皮质骨结构。研究结束时，处死大鼠并取其骨骼进行分析。HCHF 组大鼠在第 12 周建立代谢综合征。在 HCHF 组，第 20 周时观察到小梁骨明显恶化（p<0.05）。HCHF 饮食还显著降低了皮质和组织面积（p<0.05），但不影响皮质厚度和骨钙含量（p>0.05）。HCHF 组的股骨长度和宽度明显短于正常组（p<0.05）。生物力学强度测试显示，与正常大鼠相比，HCHF 大鼠的股骨能承受的力显著降低，但位移和应变显著增加（p<0.05）。总之，HCHF 饮食诱导的 MetS 可对骨骼产生不良影响。
