Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaakob Latif, Bandar Tun Razak, 56000, Cheras, Kuala Lumpur, Malaysia.
Department of Anatomy, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaakob Latif, Bandar Tun Razak, 56000, Cheras, Kuala Lumpur, Malaysia.
Bone. 2018 Nov;116:8-21. doi: 10.1016/j.bone.2018.07.003. Epub 2018 Jul 7.
Metabolic syndrome (MetS) is associated with osteoporosis due to the underlying inflammatory and hormonal changes. Annatto tocotrienol has been shown to improve medical complications associated with MetS or bone loss in animal studies. This study aimed to investigate the effects of annatto tocotrienol as a single treatment for MetS and osteoporosis in high-carbohydrate high-fat (HCHF) diet-induced MetS animals. Three-month-old male Wistar rats were randomly divided into five groups. The baseline group was euthanized at the onset of the study. The normal group received standard rat chow and tap water. The remaining groups received HCHF diet and treated with three different regimens orally daily: (a) tocopherol-stripped corn oil (the vehicle of tocotrienol), (b) 60 mg/kg annatto tocotrienol, and (c) 100 mg/kg annatto tocotrienol. At the end of the study, measurements of MetS parameters, body compositions, and bone mineral density were performed in animals before sacrifice. Upon euthanasia, blood and femur of the rats were harvested for the evaluations of bone microstructure, biomechanical strength, remodelling activities, hormonal changes, and inflammatory response. Treatment with annatto tocotrienol improved all MetS parameters (except abdominal obesity), trabecular bone microstructure, bone strength, increased osteoclast number, normalized hormonal changes and inflammatory response in the HCHF animals. In conclusion, annatto tocotrienol is a potential agent for managing MetS and osteoporosis concurrently. The beneficial effects of annatto tocotrienol may be attributed to its ability to prevent the hormonal changes and pro-inflammatory state in animals with MetS.
代谢综合征（MetS）与骨质疏松症有关，这是由于潜在的炎症和激素变化。安纳托三烯生育酚已被证明可以改善与 MetS 或骨丢失相关的医学并发症。本研究旨在探讨安纳托三烯生育酚作为单一治疗代谢综合征和骨质疏松症的效果，用于高碳水化合物高脂肪（HCHF）饮食诱导的代谢综合征动物模型。将 3 月龄雄性 Wistar 大鼠随机分为五组。基线组在研究开始时安乐死。正常组给予标准大鼠饲料和自来水。其余组给予 HCHF 饮食，并每天口服三种不同方案治疗：（a）生育酚去除玉米油（生育三烯酚的载体），（b）60mg/kg 安纳托三烯生育酚，和（c）100mg/kg 安纳托三烯生育酚。在研究结束时，在动物安乐死前对代谢综合征参数、身体成分和骨密度进行测量。安乐死后，采集大鼠的血液和股骨，用于评估骨微观结构、生物力学强度、重塑活动、激素变化和炎症反应。安纳托三烯生育酚治疗改善了 HCHF 动物的所有代谢综合征参数（除了腹部肥胖）、小梁骨微观结构、骨强度、增加破骨细胞数量、使激素变化和炎症反应正常化。总之，安纳托三烯生育酚是一种同时管理代谢综合征和骨质疏松症的潜在药物。安纳托三烯生育酚的有益作用可能归因于其预防代谢综合征动物的激素变化和促炎状态的能力。
