Duangjai Acharaporn, Nuengchamnong Nitra, Lee Learn-Han, Goh Bey-Hing, Saokaew Surasak, Suphrom Nungruthai
a Division of Physiology, School of Medical Sciences , University of Phayao , Phayao , Thailand.
b Science Laboratory Centre, Faculty of Science , Naresuan University , Phitsanulok , Thailand.
Nat Prod Res. 2019 May;33(10):1491-1494. doi: 10.1080/14786419.2017.1416386. Epub 2017 Dec 19.
has long been used in traditional medicine. This study focused on isolation and characterisation of active ingredients in the extract, its fractions (NF-EA, NF-AQ, NF-G) and its effect on the cholesterol absorption activity. The NF-EA fraction was identified by marker compounds by LC-ESI-QTOF/MS. Cholesterol absorption activity was performed by measuring the solubility and size of cholesterol micelles. The intestinal motility was also examined by isolated rat's ileum to test the contraction. The extract and its fractions consist of flavonoids and phenolic compounds, like quercetin, kaempferol and myricetin. We found that extract and NF-EA increase cholesterol micelles size, while the extract, NF-AQ, myricetin and quercetin, reduced the solubility of cholesterol in micelles. The extract and quercetin inhibited the contraction induced by KCl up to 29 and 18%, respectively, and also decreased CaCl-induced contraction. This finding is in support to traditional uses of as cholesterol-lowering agents and regulator of gastrointestinal motility.
长期以来一直被用于传统医学。本研究聚焦于提取物中活性成分的分离与表征、其馏分（NF-EA、NF-AQ、NF-G）及其对胆固醇吸收活性的影响。通过LC-ESI-QTOF/MS利用标记化合物鉴定了NF-EA馏分。通过测量胆固醇微团的溶解度和大小来进行胆固醇吸收活性实验。还通过分离大鼠回肠来检测收缩情况以研究肠道蠕动。提取物及其馏分含有黄酮类化合物和酚类化合物，如槲皮素、山奈酚和杨梅素。我们发现提取物和NF-EA会增加胆固醇微团的大小，而提取物、NF-AQ、杨梅素和槲皮素会降低胆固醇在微团中的溶解度。提取物和槲皮素分别抑制由氯化钾诱导的收缩达29%和18%，并且还降低了氯化钙诱导的收缩。这一发现支持了其作为降胆固醇药物和胃肠动力调节剂的传统用途。
