Yamauchi Aisa, Kobayashi Asami, Oikiri Hiroe, Yokoyama Yoshihito
Department of Obstetrics and Gynecology Hirosaki University Graduate School of Medicine Hirosaki Japan.
Reprod Med Biol. 2017 Mar 20;16(2):143-151. doi: 10.1002/rmb2.12019. eCollection 2017 Apr.
The microtubule-associated Tau protein is a marker of paclitaxel sensitivity in ovarian cancer. The aim of the present study was to elucidate the function of the Tau protein in epithelial ovarian cancer.
The correlation between Tau protein expression and the response to paclitaxel by using several ovarian cancer cell lines was investigated.
A Western blot showed that the expression level of the Tau protein was the highest in the TOV112D cells. A cell-counting kit showed that the proliferation rates were more inhibited in the cells with down-regulated Tau protein than in the control cells, both with and without paclitaxel treatment. The proliferation rates of the control cells and the TOV112D cells also were compared with Tau protein overexpression. The level of cell proliferation was more inhibited in the cells that overexpressed the Tau protein, compared to the control cells, both with and without paclitaxel treatment. It was shown that both the down-regulation and the overexpression of the Tau protein were related to the inhibition of TOV112D cell proliferation. Early and late apoptosis of the TOV112D cells that were transfected with Tau cDNA plasmid construct or Tau small interfering RNA significantly increased.
These findings suggest that the molecular targeting of the Tau protein could be a potential treatment for ovarian cancer.
微管相关的Tau蛋白是卵巢癌中紫杉醇敏感性的标志物。本研究的目的是阐明Tau蛋白在上皮性卵巢癌中的功能。
利用几种卵巢癌细胞系研究Tau蛋白表达与对紫杉醇反应之间的相关性。
蛋白质免疫印迹显示，Tau蛋白的表达水平在TOV112D细胞中最高。细胞计数试剂盒显示，无论有无紫杉醇处理，Tau蛋白下调的细胞中增殖率均比对照细胞受到更强抑制。还比较了对照细胞和TOV112D细胞在Tau蛋白过表达情况下的增殖率。无论有无紫杉醇处理，过表达Tau蛋白的细胞中细胞增殖水平均比对照细胞受到更强抑制。结果表明，Tau蛋白的下调和过表达均与TOV112D细胞增殖的抑制有关。用Tau cDNA质粒构建体或Tau小干扰RNA转染的TOV112D细胞的早期和晚期凋亡显著增加。
这些发现表明，Tau蛋白的分子靶向可能是卵巢癌的一种潜在治疗方法。
