基于表面增强拉曼散射的氯霉素定量灵敏检测
Quantitative and Sensitive Detection of Chloramphenicol by Surface-Enhanced Raman Scattering.
作者信息
Ding Yufeng, Zhang Xin, Yin Hongjun, Meng Qingyun, Zhao Yongmei, Liu Luo, Wu Zhenglong, Xu Haijun
机构信息
Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing 100029, China.
College of Science, Beijing University of Chemical Technology, Beijing 100029, China.
出版信息
Sensors (Basel). 2017 Dec 20;17(12):2962. doi: 10.3390/s17122962.
We used surface-enhanced Raman scattering (SERS) for the quantitative and sensitive detection of chloramphenicol (CAP). Using 30 nm colloidal Au nanoparticles (NPs), a low detection limit for CAP of 10 M was obtained. The characteristic Raman peak of CAP centered at 1344 cm was used for the rapid quantitative detection of CAP in three different types of CAP eye drops, and the accuracy of the measurement result was verified by high-performance liquid chromatography (HPLC). The experimental results reveal that the SERS technique based on colloidal Au NPs is accurate and sensitive, and can be used for the rapid detection of various antibiotics.
我们使用表面增强拉曼散射（SERS）对氯霉素（CAP）进行定量和灵敏检测。使用30纳米的胶体金纳米颗粒（NPs），获得了CAP的低检测限为10 M。CAP的特征拉曼峰位于1344 cm处，用于快速定量检测三种不同类型的CAP眼药水，测量结果的准确性通过高效液相色谱法（HPLC）进行了验证。实验结果表明，基于胶体金纳米颗粒的SERS技术准确且灵敏，可用于各种抗生素的快速检测。
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