Ji Wei, Yao Weirong
School of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, Guangdong, PR China.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Jun 5;144:125-30. doi: 10.1016/j.saa.2015.02.029. Epub 2015 Feb 16.
Chloramphenicol (CAP) is a widely used amide alcohol antibiotics, which has been banned from using in food producing animals in many countries. In this study, surface enhanced Raman scattering (SERS) coupled with gold colloidal nanoparticles was used for the rapid analysis of CAP. Density functional theory (DFT) calculations were conducted with Gaussian 03 at the B3LYP level using the 3-21G(d) and 6-31G(d) basis sets to analyze the assignment of vibrations. Affirmatively, the theoretical Raman spectrum of CAP was in complete agreement with the experimental spectrum. They both exhibited three strong peaks characteristic of CAP at 1104 cm(-1), 1344 cm(-1), 1596 cm(-1), which were used for rapid qualitative analysis of CAP residues in food samples. The use of SERS as a method for the measurements of CAP was explored by comparing use of different solvents, gold colloidal nanoparticles concentration and absorption time. The method of the detection limit was determined as 0.1 μg/mL using optimum conditions. The Raman peak at 1344 cm(-1) was used as the index for quantitative analysis of CAP in food samples, with a linear correlation of R(2)=0.9802. Quantitative analysis of CAP residues in foods revealed that the SERS technique with gold colloidal nanoparticles was sensitive and of a good stability and linear correlation, and suited for rapid analysis of CAP residue in a variety of food samples.
氯霉素（CAP）是一种广泛使用的酰胺醇类抗生素，在许多国家已被禁止用于食用动物。在本研究中，表面增强拉曼散射（SERS）结合金胶体纳米粒子用于氯霉素的快速分析。使用高斯03软件在B3LYP水平上，采用3-21G(d)和6-31G(d)基组进行密度泛函理论（DFT）计算，以分析振动归属。肯定地说，氯霉素的理论拉曼光谱与实验光谱完全一致。它们都在1104 cm(-1)、1344 cm(-1)、1596 cm(-1)处呈现出氯霉素特有的三个强峰，这些峰用于食品样品中氯霉素残留的快速定性分析。通过比较不同溶剂、金胶体纳米粒子浓度和吸附时间的使用情况，探索了将SERS作为测量氯霉素的一种方法。在最佳条件下，检测限方法确定为0.1μg/mL。以1344 cm(-1)处的拉曼峰作为食品样品中氯霉素定量分析的指标，线性相关系数R(2)=0.9802。食品中氯霉素残留的定量分析表明，金胶体纳米粒子的SERS技术灵敏，具有良好的稳定性和线性相关性，适用于多种食品样品中氯霉素残留的快速分析。
