[VDLP 方案化疗诱导缓解后 B 淋巴细胞白血病患儿外周血骨髓来源抑制细胞水平的变化及其与免疫系统的关系]

[Changes of Peripheral Blood Marrow-Derived Suppressor Cell Level after Chemotherapy Induction Remission by VDLP Regimen and Their Relationship with Immune System in B-ALL Children].

作者信息

Guo Xue-Mei, Fang Yong-Jun, Lv Cheng-Lan, Wang Yong-Ren, Sun Xiao-Yan

机构信息

Department of Hematology and Oncology, The Affiliated Children's Hospital of Nanjing Medical University, Nanjing 210008, Jiangsu Province, China.

Department of Hematology and Oncology, The Affiliated Children's Hospital of Nanjing Medical University, Nanjing 210008, Jiangsu Province, China. E-mail:

出版信息

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2017 Dec;25(6):1611-1614. doi: 10.7534/j.issn.1009-2137.2017.06.005.

DOI:10.7534/j.issn.1009-2137.2017.06.005

PMID:29262884

Abstract

OBJECTIVE

To investigate the changes of peripheral blood marrow-derived suppressor cell level after chemotherapy induction remission by regimen consisting of vincristine, daunorubicin, L-asparaginase and prednisone (VDLP) and to analyze their relationship with immume system in B-ALL children.

METHODS

Thirty B-ALL children after induction remission by VDLP regimen from August 2015 to August 2016 were selected as B-ALL group and 30 normal healthy children were selected as control group. The peripheral blood in 2 groups was collected and detected by flow cytometry, then the ratios of CD30 cells and CD33 HLA-DR marrow-derived suppressor cells, CD14CD33HLA-DR marrow-derived suppressor cells and CD15CD33HLA-DR marrow-derived suppressor cells were calculated, and their changes after induction remission by VDLP regimen and the relationship with immune system were analyzed.

RESULTS

After treatment the ratio of CD33 cells in peripheral blood of B-ALL group and control group was not significantly different (P> 0.05), moreover, the ratio of CD33 cells in B-ALL group was significantly higher than that before treatment (P<0.05), while the ratios of CD33 HLA-DR marrow-derived suppressor cells, CD14CD33HLA-DR marrow-derived suppressor cells and CD15CD33HLA-DR marrow-derived suppressor cells in B-ALL group were significantly lower than those in control group (all P<0.05), but the ratios of these cells in B-ALL group were higher than those before treatment, and yet there was no statistical significance (P>0.05).

CONCLUSION

The ratios of marrow-derived suppressor cells in peripheral blood of B-ALL children in complete remission after treatment with VDLP regimen are higher than those before treatment, but are significantly lower than normal value, which may be related with non-complese recovery of immune system in B-ALL children after treatment.

摘要

目的

探讨长春新碱、柔红霉素、L-门冬酰胺酶和泼尼松（VDLP）方案化疗诱导缓解后B淋巴细胞白血病（B-ALL）患儿外周血骨髓来源抑制细胞水平的变化，并分析其与免疫系统的关系。

方法

选取2015年8月至2016年8月采用VDLP方案诱导缓解后的30例B-ALL患儿作为B-ALL组，选取30例正常健康儿童作为对照组。采集两组外周血，采用流式细胞术进行检测，计算CD3⁺细胞、CD33⁺HLA-DR⁻骨髓来源抑制细胞、CD14⁺CD33⁺HLA-DR⁻骨髓来源抑制细胞及CD15⁺CD33⁺HLA-DR⁻骨髓来源抑制细胞的比例，分析VDLP方案诱导缓解后其变化及与免疫系统的关系。

结果

治疗后B-ALL组与对照组外周血CD33⁺细胞比例差异无统计学意义（P>0.05），且B-ALL组CD33⁺细胞比例高于治疗前（P<0.05）；B-ALL组CD33⁺HLA-DR⁻骨髓来源抑制细胞、CD14⁺CD33⁺HLA-DR⁻骨髓来源抑制细胞及CD15⁺CD33⁺HLA-DR⁻骨髓来源抑制细胞比例均低于对照组（均P<0.05），但B-ALL组这些细胞比例高于治疗前，差异无统计学意义（P>0.05）。