Whiting A, Wardale J, Trinick J
Muscle Biology Department, A.F.R.C. Institute of Food Research, Langford, Bristol, U.K.
J Mol Biol. 1989 Jan 5;205(1):263-8. doi: 10.1016/0022-2836(89)90381-1.
The protein titin has been localized by electron microscopy of myofibrils labelled with monoclonal antibodies. The data are consistent with individual titin molecules extending from near the M-line to beyond the ends of thick filaments, a distance of approximately 1 micron. In the A-band, titin appears to be bound to thick filaments, probably to the outside of the filament shaft. Molecules of titin in this configuration provided an obvious mechanism by which the length of thick filaments could be regulated accurately.
通过用单克隆抗体标记肌原纤维的电子显微镜技术，已将肌联蛋白定位。数据表明单个肌联蛋白分子从M线附近延伸至粗肌丝末端之外，距离约为1微米。在A带中，肌联蛋白似乎与粗肌丝结合，可能是在肌丝轴的外侧。处于这种构型的肌联蛋白分子提供了一种明显的机制，通过该机制可以精确调节粗肌丝的长度。
