鉴定结直肠癌细胞中甲基硫腺苷磷酸化酶（MTAP）的表达。

Characterization of methylthioadenosin phosphorylase (MTAP) expression in colorectal cancer.

机构信息

a Department of Gastroenterology , Weifang Peoples' Hospital , Weifang , Shandong , China.

b Department of Anesthesiology , Affiliated Hospital of Weifang Medical University , Weifang , China.

出版信息

Artif Cells Nanomed Biotechnol. 2018 Dec;46(8):2082-2087. doi: 10.1080/21691401.2017.1408122. Epub 2017 Dec 21.

DOI:10.1080/21691401.2017.1408122

PMID:29268653

Abstract

PURPOSE

Tumour seriously affects people's quality of life. Colorectal cancer is a refractory tumour in digestive tract tumors. In colorectal cancer, gene expression abnormalities is the main reason for its incidence, we mainly focus on the molecular mechanism of MTAP in the development of colorectal cancer.

METHODS

The tumour tissue and its adjacent tissue samples of 50 patients with colorectal cancer were screened from July 2011 to February 2015, and the expression of MTAP was detected. Cell lines that overexpress MTAP and low expression of MTAP were constructed in colorectal cancer cell lines. The cell proliferation, invasion and migration was detected in the cells with different expression levels of MTAP. Immunohistochemistry was used to detect the expression of MTAP in liver metastasis and to investigate its clinical significance. And statistics of clinical significance.

RESULTS

Q-PCR results showed that the expression of MTAP in colorectal cancer cell lines were significantly higher than that normal human colonic myofibroblasts cell line. Cell proliferation test results showed that cell proliferation was accelerated when MTAP was overexpression, cell invasion and migration were simultaneously accelerated. The expression of MTAP in primary liver was positively correlated with metastatic disease in patients with liver metastatic colorectal cancer via EMT.

CONCLUSIONS

MTAP accelerates the growth and metastasis of colorectal cancer through EMT.

摘要

目的

肿瘤严重影响人们的生活质量。结直肠癌是消化道肿瘤中的一种难治性肿瘤。在结直肠癌中，基因表达异常是其发病的主要原因，我们主要关注 MTAP 在结直肠癌发生发展中的分子机制。

方法

从 2011 年 7 月至 2015 年 2 月，筛选了 50 例结直肠癌患者的肿瘤组织及其相邻组织标本，检测 MTAP 的表达。在结直肠癌细胞系中构建 MTAP 过表达和低表达的细胞系。检测不同 MTAP 表达水平的细胞中的细胞增殖、侵袭和迁移。用免疫组化检测 MTAP 在肝转移中的表达，探讨其临床意义。并对临床意义进行统计学分析。