Hasegawa Ayana, Watanabe Miwa, Osada Hironari, Ogawa Misato, Ohno Hikaru, Yanuma Nanako, Sasaki Kazuaki, Shimoda Minoru, Shirai Junsuke, Ohmori Keitaro
Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan.
Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan.
Biochem Biophys Res Commun. 2018 Jan 15;495(3):2184-2188. doi: 10.1016/j.bbrc.2017.12.099. Epub 2017 Dec 19.
A time-of-day-dependent variation in IgE-mediated passive systemic anaphylaxis was previously reported in ICR mice. In the present study, we investigated time-of-day-dependent variations in IgE-, histamine-, and platelet-activating factor (PAF)-mediated systemic anaphylaxis in C57BL/6, BALB/c, and NC/Nga mice at 9:00 h and 21:00 h, and evaluated the potential influence of glucocorticoids (GCs) on these variations. We found significant time-of-day-dependent variations in IgE-mediated systemic anaphylaxis in C57BL/6 mice, and in histamine- and PAF-mediated systemic anaphylaxis in BALB/c mice. Significant daily variations in IgE-, histamine-, and PAF-mediated systemic anaphylaxis were not observed in NC/Nga mice. Pretreatment with dexamethasone and adrenalectomy abolished the daily variations in IgE-mediated systemic anaphylaxis in C57BL/6 mice and in PAF-mediated systemic anaphylaxis in BALB/c mice, suggesting that GCs from adrenal glands are pivotal in regulating these variations. In contrast, pretreatment with dexamethasone and adrenalectomy did not abolish the daily variation in histamine-mediated systemic anaphylaxis in BALB/c mice, suggesting that GC-independent and adrenal gland-independent mechanisms are important for the variation. The present study demonstrated that time-of-day-dependent variations in systemic anaphylaxis differed among inbred mouse strains and with anaphylaxis-inducing substances. Thus, mouse strains, time of experiment, and anaphylaxis-inducing substances used must be considered to obtain appropriate experimental results.
先前有报道称，ICR小鼠中存在IgE介导的被动全身过敏反应的昼夜变化。在本研究中，我们调查了C57BL/6、BALB/c和NC/Nga小鼠在9:00和21:00时IgE、组胺和血小板活化因子（PAF）介导的全身过敏反应的昼夜变化，并评估了糖皮质激素（GCs）对这些变化的潜在影响。我们发现C57BL/6小鼠中IgE介导的全身过敏反应以及BALB/c小鼠中组胺和PAF介导的全身过敏反应存在显著的昼夜变化。在NC/Nga小鼠中未观察到IgE、组胺和PAF介导的全身过敏反应的显著每日变化。地塞米松预处理和肾上腺切除术消除了C57BL/6小鼠中IgE介导的全身过敏反应以及BALB/c小鼠中PAF介导的全身过敏反应的每日变化，表明来自肾上腺的GCs在调节这些变化中起关键作用。相比之下，地塞米松预处理和肾上腺切除术并未消除BALB/c小鼠中组胺介导的全身过敏反应的每日变化，表明GC非依赖性和肾上腺非依赖性机制对该变化很重要。本研究表明，全身过敏反应的昼夜变化在近交系小鼠品系之间以及与过敏反应诱导物质有关的方面存在差异。因此，为了获得合适的实验结果，必须考虑小鼠品系、实验时间和所使用的过敏反应诱导物质。
