Isogai E, Isogai H, Miura H, Takano K, Aoi Y, Hayashi M, Namioka S
Nihon Juigaku Zasshi. 1989 Feb;51(1):110-8. doi: 10.1292/jvms1939.51.110.
The plaque flora was studied in adult mongrel and beagle dogs with periodontal disease. Gingival plaque from maxillary premolars was removed and cultured on various growth media. The flora in all dogs was composed of mostly anaerobic gram negative rods. Bacteroides asaccharolyticus was found in the highest proportion of plaque samples from mongrel dogs, and decomposed hydrogen peroxide suggesting catalase activity. Fusobacterium nucleatum was found in higher proportion in the plaque of beagle dogs as compared to B. asaccharolyticus. With the increasing numbers of obligative anaerobic gram negative organisms such as B. asaccharolyticus, the proportions of Streptococcus, Enterococcus and Staphylococcus decreased in the dogs with periodontal disease. The salivary flora was different from the plaque flora of the dogs with periodontal disease. It was constant regardless with the disease. The salivary flora of beagle dogs with the healthy gingiva was different from that of mongrel dogs. Enterococcus, Lactobacillus, Eubacterium and black-pigmented Bacteroides (BPB, mainly B. asaccharolyticus) were higher proportion in the flora of beagle dogs as compared to mongrel dogs, while Fusobacterium, Enterobacteriaceae, yeast and molds were lower in the flora. The results reveal that B. asaccharolyticus and F. nucleatum are common pathogens and uniquely contribute to the development of gingival inflammation in dog.
对患有牙周病的成年杂种犬和比格犬的菌斑菌群进行了研究。从上颌前磨牙采集牙龈菌斑，并在各种生长培养基上进行培养。所有犬的菌群主要由厌氧革兰氏阴性杆菌组成。在杂种犬的菌斑样本中，无糖分解拟杆菌的比例最高，且该菌能分解过氧化氢，提示有过氧化氢酶活性。与无糖分解拟杆菌相比，具核梭杆菌在比格犬菌斑中的比例更高。随着诸如无糖分解拟杆菌等 obligative 厌氧革兰氏阴性菌数量的增加，患牙周病犬的链球菌、肠球菌和葡萄球菌比例下降。唾液菌群与患牙周病犬的菌斑菌群不同。无论疾病情况如何，其都保持恒定。牙龈健康的比格犬的唾液菌群与杂种犬不同。与杂种犬相比，肠球菌、乳酸杆菌、真杆菌和产黑色素拟杆菌（BPB，主要是无糖分解拟杆菌）在比格犬菌群中的比例更高，而梭杆菌、肠杆菌科、酵母和霉菌在菌群中的比例更低。结果表明，无糖分解拟杆菌和具核梭杆菌是常见病原体，对犬牙龈炎症的发展有独特作用。
