Yin Zhi-Nang, Ding Jin-Li, Zhang Yi, Li Sai-Jiao, Zhang Yan, Yang Jing
Department of Histology and Embryology, Medical College of Wuhan University, Wuhan, 430071, China.
Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
J Huazhong Univ Sci Technolog Med Sci. 2017 Dec;37(6):910-914. doi: 10.1007/s11596-017-1826-5. Epub 2017 Dec 21.
E2A is involved in promoting forkhead box P3 (FOXP3) and retinoid-related orphan receptor gamma t (RORγt) gene transcription, which are pivotal transcription factors of T regulatory cells and Th17 cells, respectively. Little is known about the involvement of E2A in pregnancy process. This study aimed to investigate the expression of E2A, cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), and Foxp3 in luteal phase endometrium of women suffering recurrent miscarriage (RM) (n=21) and control group (n=11) by immunohistochemistry, with the Vectra® automated quantitative pathology imaging system for analysis. The percentage of E2A+ cells and CTLA-4+ cells was significantly higher in the endometrium of women with RM than in the controls. There was positive correlation between E2A and CTLA-4 (r=0.523, P=0.002), E2A and FOXP3 (r=0.380, P=0.032), and FOXP3 and CTLA-4 (r=0.625, P=0.000) in the mid-secretory phase of endometrium for all subjects. It was concluded that the abnormal expression of endometrial E2A existed in mid-secretory endometrium of women with RM, and there was a positive correlation between E2A and FOXP3, and E2A and CTLA-4, suggesting the possible regulation role of E2A involved in regulating endometrium receptivity.
E2A参与促进叉头框蛋白P3（FOXP3）和维甲酸相关孤儿受体γt（RORγt）基因转录，它们分别是调节性T细胞和Th17细胞的关键转录因子。关于E2A在妊娠过程中的作用知之甚少。本研究旨在通过免疫组织化学方法，使用Vectra®自动定量病理成像系统分析，调查复发性流产（RM）患者（n = 21）和对照组（n = 11）黄体期子宫内膜中E2A、细胞毒性T淋巴细胞相关蛋白4（CTLA - 4）和Foxp3的表达情况。RM患者子宫内膜中E2A +细胞和CTLA - 4 +细胞的百分比显著高于对照组。在所有受试者子宫内膜的分泌中期，E2A与CTLA - 4（r = 0.523，P = 0.002）、E2A与FOXP3（r = 0.380，P = 0.032）以及FOXP3与CTLA - 4（r = 0.625，P = 0.000）之间存在正相关。研究得出结论，RM患者分泌中期子宫内膜存在E2A异常表达，且E2A与FOXP3、E2A与CTLA - 4之间存在正相关，提示E2A可能参与调节子宫内膜容受性。
