Reis M G, Kuhns J, Blanton R, Davis A H
Escola de Medicina e Saude Publica, Salvador Bahia, Brazil.
Mol Biochem Parasitol. 1989 Jan 15;32(2-3):113-9. doi: 10.1016/0166-6851(89)90062-5.
To understand mechanisms involved in sex-specific gene expression in Schistosoma mansoni, a cDNA (fs800) was isolated that hybridized to an 800 nucleotide mRNA present in high levels only in mature female worms. The fs800 cDNA sequence was characterized by two long open reading frames and central stretches of repeated amino acids. Fs800 did not share similarities with other known sequences in computer searches. In situ hybridization, however, revealed that the mRNA corresponding to fs800 was found only in female vitelline cells, suggesting that the product of this gene may be involved in the production or function of eggs. Fs800 is developmentally regulated as expression of this gene is dependent on the maturity of female worms. Furthermore, during in vitro culture, when female worms are known to stop egg production, expression of fs800 selectively ceased.
为了解曼氏血吸虫性别特异性基因表达所涉及的机制，分离出了一个与仅在成熟雌虫中高水平存在的800个核苷酸的mRNA杂交的cDNA（fs800）。fs800 cDNA序列的特征是有两个长开放阅读框和中央重复氨基酸序列。在计算机搜索中，Fs800与其他已知序列没有相似性。然而，原位杂交显示，与fs800对应的mRNA仅在雌性卵黄细胞中发现，这表明该基因的产物可能参与卵的产生或功能。Fs800受发育调控，因为该基因的表达取决于雌虫的成熟度。此外，在体外培养期间，已知雌虫停止产卵时，fs800的表达选择性地停止。
