Guan Qijie, Yu Jiaojiao, Zhu Wei, Yang Bingxian, Li Yaohan, Zhang Lin, Tian Jingkui
College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou 310027, PR China.
School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 10081, PR China.
Gene. 2018 Mar 1;645:60-68. doi: 10.1016/j.gene.2017.12.045. Epub 2017 Dec 21.
Ultraviolet-B (UVB) irradiation induces oxidative stress in plant cells due to the generation of excessive reactive oxygen species. Morus alba L. (M. abla) is an important medicinal plant used for the treatment of human diseases. Also, its leaves are widely used as food for silkworms. In our previous research, we found that a high level of UVB irradiation with dark incubation led to the accumulation of secondary metabolites in M. abla leaf. The aim of the present study was to describe and compare M. alba leaf transcriptomics with different treatments (control, UVB, UVB+dark). Leaf transcripts from M. alba were sequenced using an Illumina Hiseq 2000 system, which produced 14.27Gb of data including 153,204,462 paired-end reads among the three libraries. We de novo assembled 133,002 transcripts with an average length of 1270bp and filtered 69,728 non-redundant unigenes. A similarity search was performed against the non-redundant National Center of Biotechnology Information (NCBI) protein database, which returned 41.08% hits. Among the 20,040 unigenes annotated in UniProtKB/SwissProt database, 16,683 unigenes were assigned 102,232 gene ontology terms and 6667 unigenes were identified in 287 known metabolic pathways. Results of differential gene expression analysis together with real-time quantitative PCR tests indicated that UVB irradiation with dark incubation enhanced the flavonoid biosynthesis in M. alba leaf. Our findings provided a valuable proof for a better understanding of the metabolic mechanism under abiotic stresses in M. alba leaf.
紫外线B（UVB）照射会因产生过量活性氧而在植物细胞中诱导氧化应激。桑树（Morus alba L.）是一种用于治疗人类疾病的重要药用植物。此外，其叶子还被广泛用作蚕的食物。在我们之前的研究中，我们发现高强度UVB照射并进行黑暗培养会导致桑叶中次生代谢产物的积累。本研究的目的是描述和比较不同处理（对照、UVB、UVB+黑暗）下的桑叶转录组学。使用Illumina Hiseq 2000系统对桑叶转录本进行测序，三个文库共产生了14.27Gb数据，包括153,204,462对末端读段。我们从头组装了133,002个转录本，平均长度为1270bp，并筛选出69,728个非冗余单基因。对非冗余的美国国立生物技术信息中心（NCBI）蛋白质数据库进行相似性搜索，命中率为41.08%。在UniProtKB/SwissProt数据库中注释的20,040个单基因中，16,683个单基因被赋予了102,232个基因本体论术语，6667个单基因在287条已知代谢途径中被鉴定出来。差异基因表达分析结果以及实时定量PCR测试表明，UVB照射并进行黑暗培养增强了桑叶中黄酮类化合物的生物合成。我们的研究结果为更好地理解桑叶在非生物胁迫下的代谢机制提供了有价值的证据。
