Krivova Yuliya S, Proshchina Alexandra E, Barabanov Valeriy M, Barinova Irina V, Saveliev Sergey V
Laboratory of Nervous System Development, Research Institute of Human Morphology, 3 Tsurupy St., Moscow 117418, Russia.
Laboratory of Nervous System Development, Research Institute of Human Morphology, 3 Tsurupy St., Moscow 117418, Russia.
Early Hum Dev. 2018 Feb;117:44-49. doi: 10.1016/j.earlhumdev.2017.12.009. Epub 2017 Dec 21.
Expression of the intermediate filament protein vimentin has been recently observed in the pancreatic islet β- and α-cells of humans with type 2 diabetes mellitus. It was suggested that the presence of vimentin in endocrine cells may indicate islet tissue renewal, or potentially represent the dedifferentiation of endocrine cells, which could contribute to the onset of type 2 diabetes or islet cell dysfunction.
To analyze the expression of vimentin in pancreatic β- and α-cells of macrosomic infants of diabetic and nondiabetic mothers.
Pancreatic samples of five macrosomic infants (gestational age 34-40weeks) from three diabetic and two nondiabetic mothers were compared to six control infants (32-40weeks, weight appropriate for gestational age) from normoglycemic mothers.
Pancreatic autopsy samples were examined by double immunofluorescent labeling with antibodies against vimentin and either insulin or glucagon. Alterations in the endocrine pancreas were measured using morphometric methods, then data were statistically analyzed.
In the pancreatic islets of macrosomic infants from diabetic and nondiabetic mothers, we observed vimentin-positive cells, some of which simultaneously contained insulin or glucagon. We also quantitatively showed that the presence of such cells was associated with hypertrophy and hyperplasia of the islets, and with an increase in β- and α-cell density.
We speculate that the appearance of vimentin-positive islet cells may reflect induction of differentiation in response to the increased insulin demand, and vimentin may serve as an early marker of endocrine pancreas disorders.
最近在2型糖尿病患者的胰岛β细胞和α细胞中观察到中间丝蛋白波形蛋白的表达。有人认为内分泌细胞中波形蛋白的存在可能表明胰岛组织更新，或者可能代表内分泌细胞的去分化，这可能导致2型糖尿病的发生或胰岛细胞功能障碍。
分析糖尿病母亲和非糖尿病母亲所生巨大儿的胰腺β细胞和α细胞中波形蛋白的表达。
将3名糖尿病母亲和2名非糖尿病母亲所生的5名巨大儿（胎龄34 - 40周）的胰腺样本与6名血糖正常母亲所生的对照婴儿（32 - 40周，体重与胎龄相符）的胰腺样本进行比较。
用抗波形蛋白和胰岛素或胰高血糖素的抗体进行双重免疫荧光标记，检查胰腺尸检样本。使用形态计量学方法测量内分泌胰腺的变化，然后对数据进行统计分析。
在糖尿病母亲和非糖尿病母亲所生巨大儿的胰岛中，我们观察到波形蛋白阳性细胞，其中一些同时含有胰岛素或胰高血糖素。我们还定量显示，这些细胞的存在与胰岛肥大、增生以及β细胞和α细胞密度增加有关。
我们推测波形蛋白阳性胰岛细胞的出现可能反映了对胰岛素需求增加的分化诱导，并且波形蛋白可能作为内分泌胰腺疾病的早期标志物。
