a Department of Biophysics, Faculty of Medicine , Karadeniz Technical University , Trabzon , Turkey.
b Department of Medical Biochemistry, Faculty of Medicine , Karadeniz Technical University , Trabzon , Turkey.
Arch Physiol Biochem. 2018 Dec;124(5):442-447. doi: 10.1080/13813455.2017.1420666. Epub 2017 Dec 25.
In this study we aimed to investigate whether reduced BDNF levels aggravate the susceptibility of the brain to hazardous effects of high fat diet. For this purpose, we fed BDNF heterozygous mice and wild type littermates with normal and high fat diet for 16 weeks. Concentrations of two synaptic proteins (SNAP-25 and PSD-95) and oxidative stress parameters (MDA, SOD, CAT) were evaluated in the cortex after diet period. Interestingly, body weights of BDNF heterozygous groups fed with control diet were higher than their littermates and heterozygous mice fed with HFD were the heaviest in all experimental groups. MDA levels were significantly elevated in both HFD groups (wild type and BDNF(+/-)). Synaptic markers PSD-95 and SNAP-25 markedly decreased in BDNF(+/-) group fed with HFD compared to other groups. In conclusion, we suggest that endogenous BDNF has an important and possibly protective role in diet-induced changes in the cortex.
在这项研究中，我们旨在探讨 BDNF 水平降低是否会加重大脑对高脂肪饮食有害影响的易感性。为此，我们用正常饮食和高脂肪饮食喂养 BDNF 杂合子小鼠和野生型同窝仔鼠 16 周。在饮食期后，评估皮质中两种突触蛋白（SNAP-25 和 PSD-95）和氧化应激参数（MDA、SOD、CAT）的浓度。有趣的是，喂食对照饮食的 BDNF 杂合子组的体重高于其同窝仔鼠，而喂食高脂肪饮食的杂合子小鼠在所有实验组中最重。两种高脂肪饮食组（野生型和 BDNF(+/-)）的 MDA 水平均显著升高。与其他组相比，喂食高脂肪饮食的 BDNF(+/-)组的突触标记物 PSD-95 和 SNAP-25 明显减少。总之，我们认为内源性 BDNF 在饮食诱导的皮质变化中具有重要的、可能具有保护作用。
