Kim Woosuk, Kwon Hyun Jung, Jung Hyo Young, Lim Soon-Sung, Kang Beom-Goo, Jo Yong-Bok, Yu Dong-Sool, Choi Soo Young, Hwang In Koo, Kim Dae Won
Department of Biomedical Sciences, Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon 24252, Korea.
Department of Anatomy, College of Veterinary Medicine, Veterinary Science Research Institute, Konkuk University, Seoul 05030, Korea.
Plants (Basel). 2021 Aug 31;10(9):1814. doi: 10.3390/plants10091814.
We investigated the effects of leaf extract (CVE) on a high-fat diet (HFD)-induced obesity and memory deficits. Male mice (5 weeks of age) were fed vehicle (distilled water), or 30, 100, or 300 mg/kg of CVE once a day for 8 weeks with an HFD. Treatment with CVE resulted in lower body weight and glucose levels in a concentration- and feeding time-dependent manner. LDL cholesterol and triglyceride levels were significantly lower in the CVE-treated HFD group than in the vehicle-treated HFD group. In contrast, high-density lipoprotein cholesterol levels did not show any significant changes. Lipid droplets and ballooning were reduced depending on the concentration of CVE treatment compared to the HFD group. Treatment with CVE ameliorated the increase in glucagon and immunoreactivities in the pancreas, and novel object recognition memory was improved by 300 mg/kg CVE treatment compared to the HFD group. More proliferating cells and differentiated neuroblasts were higher in mice treated with CVE than in vehicle-treated HFD-fed mice. Brain-derived neurotrophic factor (BDNF) levels were significantly decreased in the HFD group, which was facilitated by treatment with 300 mg/kg CVE in hippocampal homogenates. These results suggest that CVE ameliorates HFD-induced obesity and memory deficits in mice, associated with increased BDNF levels in the hippocampus.
我们研究了叶提取物（CVE）对高脂饮食（HFD）诱导的肥胖和记忆缺陷的影响。雄性小鼠（5周龄）每天一次给予溶剂（蒸馏水）或30、100或300 mg/kg的CVE，同时给予高脂饮食，持续8周。CVE治疗导致体重和血糖水平以浓度和喂养时间依赖性方式降低。与溶剂处理的高脂饮食组相比，CVE处理的高脂饮食组的低密度脂蛋白胆固醇和甘油三酯水平显著降低。相比之下，高密度脂蛋白胆固醇水平没有显示出任何显著变化。与高脂饮食组相比，根据CVE处理的浓度，脂滴和气球样变减少。CVE治疗改善了胰腺中胰高血糖素的增加和免疫反应性，与高脂饮食组相比，300 mg/kg CVE治疗改善了新物体识别记忆。与溶剂处理的高脂饮食喂养小鼠相比，CVE处理的小鼠中有更多的增殖细胞和分化的神经母细胞。高脂饮食组脑源性神经营养因子（BDNF）水平显著降低，海马匀浆中300 mg/kg CVE处理促进了这种降低。这些结果表明，CVE改善了高脂饮食诱导的小鼠肥胖和记忆缺陷，这与海马中BDNF水平升高有关。
